Vitrification of Human Spermatozoa Without Cryoprotectants

Vitrification of Human Spermatozoa Without Cryoprotectants

The aim of our study was to investigate the effects of vitrification (cooling rate ~10000°C/min) without cryoprotectants on swim-up prepared human spermatozoa in comparison to standard conventional freezing with cryoprotectants. Motility, morphology, rate of viability and acrosome reaction of sperma...

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Veröffentlicht in:Cryo-Letters 2002-03, Vol.23 (2), p.93-102
Hauptverfasser: Nawroth, F, Isachenko, V, Dessole, S, Rahimi, G, Farina, M, Vargiu, N, Mallmann, P, Dattena, M, Capobianco, G, Peters, D, Orth, I, Isachenko, E
Format: Artikel
Sprache:eng
Schlagworte:
Cell Survival
Conventional Freezing
Cryopreservation - methods
Cryoprotective Agents
Female
Fertilization in Vitro
Human Spermatozoa
Humans
Male
Nitrogen
Pregnancy
Semen Preservation - methods
Sperm Motility
Spermatozoa - cytology
VITRIFICATION
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Beschreibung
Zusammenfassung:The aim of our study was to investigate the effects of vitrification (cooling rate ~10000°C/min) without cryoprotectants on swim-up prepared human spermatozoa in comparison to standard conventional freezing with cryoprotectants. Motility, morphology, rate of viability and acrosome reaction of spermatozoa were evaluated. The described method of cryopreservation of human spermatozoa by direct plunging into liquid nitrogen slush without cryoprotectants was effective and could be recommended for routine IVF.
ISSN:0143-2044