Plaque Forming Cell Assay to Measure Responses in Mice to the Random Copolymer (Glu60Phe40)

A plaque-forming cell (PFC) assay to measure the immune response of mice to the synthetic random copolymer of glutamic acid and phenylalanine (GPhe) is described here. GPhe can be coupled to sheep red blood cells (SRBC) using "aged" CrCl3. Both IgM and IgG plaques are detected in the murin...

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Veröffentlicht in:	Immunological communications 1979, Vol.8 (5-6), p.531-538
Hauptverfasser:	Babu, U. M., Maurer, P. H., Merryman, C. F., Anderson, J. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Erythrocytes - immunology Genes, MHC Class II H-2 Antigens Hemagglutination Tests Hemolytic Plaque Technique Immunoglobulin G Immunoglobulin M Kinetics Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Peptides - immunology Phenylalanine - analogs & derivatives Polyglutamic Acid Serum Albumin, Bovine - immunology Sheep Time Factors
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Zusammenfassung:	A plaque-forming cell (PFC) assay to measure the immune response of mice to the synthetic random copolymer of glutamic acid and phenylalanine (GPhe) is described here. GPhe can be coupled to sheep red blood cells (SRBC) using "aged" CrCl3. Both IgM and IgG plaques are detected in the murine GPhe response.3 Mice of H-2 haplotypes p and q are high responders, a and k are medium or low responders and b, d and s are nonresponders as detected by the PFC assay.
ISSN:	0882-0139 0090-0877 1532-4311
DOI:	10.3109/08820137909063251