Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis

We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2002-03, Vol.99 (5), p.2690-2695
Hauptverfasser:	Drew, David, Sjöstrand, Dan, Nilsson, Johan, Urbig, Thomas, Chin, Chen-ni, de Gier, Jan-Willem, von Heijne, Gunnar
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase Antibodies Bacterial Proteins - metabolism Bioinformatics Biological Sciences Cyclin-Dependent Kinases - genetics Cyclin-Dependent Kinases - metabolism Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins Fluorescence Gels Genes, Reporter Green Fluorescent Proteins Luminescent Proteins - genetics Luminescent Proteins - metabolism MarC protein Medicin och hälsovetenskap Membrane proteins Membrane Proteins - metabolism Periplasm Phosphatases Plasmids Proteins PstA protein Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism TatC protein Time Factors Topology YaeL protein YcbM protein YddO protein YdgE protein YedZ protein YgjV protein YiaB protein YigG protein YnfA protein
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