Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis

We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2002-03, Vol.99 (5), p.2690-2695
Hauptverfasser: Drew, David, Sjöstrand, Dan, Nilsson, Johan, Urbig, Thomas, Chin, Chen-ni, de Gier, Jan-Willem, von Heijne, Gunnar
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Sprache:eng
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Zusammenfassung:We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the ≈800 inner-membrane proteins thought to exist in E. coli.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.052018199