Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis
We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2002-03, Vol.99 (5), p.2690-2695 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Drew, David Sjöstrand, Dan Nilsson, Johan Urbig, Thomas Chin, Chen-ni de Gier, Jan-Willem von Heijne, Gunnar |
| description | We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the ≈800 inner-membrane proteins thought to exist in E. coli. |
| doi_str_mv | 10.1073/pnas.052018199 |
| format | Article |
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We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the ≈800 inner-membrane proteins thought to exist in E. coli.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.052018199</identifier><identifier>PMID: 11867724</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Alkaline Phosphatase ; Antibodies ; Bacterial Proteins - metabolism ; Bioinformatics ; Biological Sciences ; Cyclin-Dependent Kinases - genetics ; Cyclin-Dependent Kinases - metabolism ; Escherichia coli ; Escherichia coli - metabolism ; Escherichia coli Proteins ; Fluorescence ; Gels ; Genes, Reporter ; Green Fluorescent Proteins ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; MarC protein ; Medicin och hälsovetenskap ; Membrane proteins ; Membrane Proteins - metabolism ; Periplasm ; Phosphatases ; Plasmids ; Proteins ; PstA protein ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; TatC protein ; Time Factors ; Topology ; YaeL protein ; YcbM protein ; YddO protein ; YdgE protein ; YedZ protein ; YgjV protein ; YiaB protein ; YigG protein ; YnfA protein</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2002-03, Vol.99 (5), p.2690-2695</ispartof><rights>Copyright 1993-2002 National Academy of Sciences of the United States of America</rights><rights>Copyright © 2002, The National Academy of Sciences 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-a4a2766ece79b2732a1fcc57923e3c87fe998d1cb153616629f3cd88e13333203</citedby><cites>FETCH-LOGICAL-c538t-a4a2766ece79b2732a1fcc57923e3c87fe998d1cb153616629f3cd88e13333203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/99/5.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3058010$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3058010$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,552,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11867724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1933678$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Drew, David</creatorcontrib><creatorcontrib>Sjöstrand, Dan</creatorcontrib><creatorcontrib>Nilsson, Johan</creatorcontrib><creatorcontrib>Urbig, Thomas</creatorcontrib><creatorcontrib>Chin, Chen-ni</creatorcontrib><creatorcontrib>de Gier, Jan-Willem</creatorcontrib><creatorcontrib>von Heijne, Gunnar</creatorcontrib><title>Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. 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We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the ≈800 inner-membrane proteins thought to exist in E. coli.</description><subject>Alkaline Phosphatase</subject><subject>Antibodies</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bioinformatics</subject><subject>Biological Sciences</subject><subject>Cyclin-Dependent Kinases - genetics</subject><subject>Cyclin-Dependent Kinases - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Fluorescence</subject><subject>Gels</subject><subject>Genes, Reporter</subject><subject>Green Fluorescent Proteins</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>MarC protein</subject><subject>Medicin och hälsovetenskap</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - metabolism</subject><subject>Periplasm</subject><subject>Phosphatases</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>PstA protein</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>TatC protein</subject><subject>Time Factors</subject><subject>Topology</subject><subject>YaeL protein</subject><subject>YcbM protein</subject><subject>YddO protein</subject><subject>YdgE protein</subject><subject>YedZ protein</subject><subject>YgjV protein</subject><subject>YiaB protein</subject><subject>YigG protein</subject><subject>YnfA protein</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqFkk1v1DAQhiMEokvhygmQT9yy9UfijwOHVdVtK7VihcrZchxn1yWxg50U9t_X0YallZCwZHk07_OOxvZk2XsElwgyctY7FZewxBBxJMSLbIGgQDktBHyZLSDELOcFLk6yNzHeQwhFyeHr7AQhThnDxSLrvqne1uDO97712z24VX1v3Rb4BlxEvTPB6p1VQPvWgmvnTMhvTVcF5QzYBD8Y6yKo9ik2tdWD9Q4oV4PNzq_OLtcbsB7jlFs51e6jjW-zV41qo3k3n6fZ9_XF3flVfvP18vp8dZPrkvAhV4XCjFKjDRMVZgQr1GhdMoGJIZqzxgjBa6QrVBKKKMWiIbrm3CCSFobkNGOHuvGX6cdK9sF2KuylVzbFvpZz_oedtoxGIkEIZTw5vxycSe5MrY0bgmqfF3imOLuTW_8gEcYFFMn_efYH_3M0cZCdjdq0bXoxP0bJUCEYLOh_QcTTE2A-VVweQB18jME0x2YQlNMMyGkG5HEGkuHT0yv8xedPT8DHGZiMf2QhZCkxFfBJa__UZTO27WB-Dwn8cADv4-DDkSQwTRmC5BEaGNAI</recordid><startdate>20020305</startdate><enddate>20020305</enddate><creator>Drew, David</creator><creator>Sjöstrand, Dan</creator><creator>Nilsson, Johan</creator><creator>Urbig, Thomas</creator><creator>Chin, Chen-ni</creator><creator>de Gier, Jan-Willem</creator><creator>von Heijne, Gunnar</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>20020305</creationdate><title>Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis</title><author>Drew, David ; 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| source | MEDLINE; SWEPUB Freely available online; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alkaline Phosphatase Antibodies Bacterial Proteins - metabolism Bioinformatics Biological Sciences Cyclin-Dependent Kinases - genetics Cyclin-Dependent Kinases - metabolism Escherichia coli Escherichia coli - metabolism Escherichia coli Proteins Fluorescence Gels Genes, Reporter Green Fluorescent Proteins Luminescent Proteins - genetics Luminescent Proteins - metabolism MarC protein Medicin och hälsovetenskap Membrane proteins Membrane Proteins - metabolism Periplasm Phosphatases Plasmids Proteins PstA protein Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism TatC protein Time Factors Topology YaeL protein YcbM protein YddO protein YdgE protein YedZ protein YgjV protein YiaB protein YigG protein YnfA protein |
| title | Rapid Topology Mapping of Escherichia coli Inner-Membrane Proteins by Prediction and PhoA/GFP Fusion Analysis |
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