Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts
Artemia salina L. (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. In this study the Medium Lethal Concentrations (LC 50 value) of 20 plant extracts, Aloe vera (L.) Burm. F. (Aloeaceae), Artemisia absinthium...
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Veröffentlicht in: | Phytomedicine (Stuttgart) 2001-09, Vol.8 (5), p.395-400 |
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Zusammenfassung: | Artemia salina L. (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. In this study the Medium Lethal Concentrations (LC
50 value) of 20 plant extracts,
Aloe vera (L.) Burm. F. (Aloeaceae),
Artemisia absinthium L. (Asteraceae);
Citrus aurantium L. (Rutaceae);
Cymbopogon citratus (DC. Ex Nees) Stapf (Poaceae);
Datura stramonium L. (Solanaceae);
Justicia pectoralis Jacq. (Acanthaceae);
Musa x paradisiaca L. (Musaceae);
Ocimum basilicum L.;
O. gratissimum L.;
O. tenuiflorum L. (Lamiaceae);
Pimenta dioica (L.) Merr. (Myrtaceae);
Piper auritum Kunth (Piperaceae);
Plantago major L. (Plantaginaceae);
Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae);
Ruta graveolens L. (Rutaceae);
Senna alata (L.) Roxb. (Fabaceae);
Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae); and
Thuja occidentalis L. (Cupressaceae), were determined using
Artemia salina L. (Artemiidae), with the objective of relating the results to the LD
50 values reported in mice (tested at three concentrations: 10, 100, and 1000 μg/mL, for each extract). We found good correlation between the
in vivo and the
in vitro tests (r = 0.85 p < 0.05), and this method is a useful tool for predicting oral acute toxicity in plant extracts. |
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ISSN: | 0944-7113 1618-095X |
DOI: | 10.1078/0944-7113-00044 |