Shear stress regulation of endothelial NOS in fetal pulmonary arterial endothelial cells involves PKC

Departments of 1 Pediatrics and 3 Molecular Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611-3008; and 2 Department of Pediatrics, University of California, San Francisco, California 94143-0106 We have shown that increased pulmonary blood flow at birth increases the activity and expression of endothelial nitric oxide (NO) synthase (eNOS). However, the signal transduction pathway regulating this process is unclear. Because protein kinase C (PKC) has been shown to be activated in response to shear stress, we undertook a study to examine its role in mediating shear stress effects on eNOS. Initial experiments demonstrated that PKC activity increased in response to shear stress. NO production in response to shear stress was found to be biphasic, with an increase in NO release up to 1 h, a plateau phase until 4 h, and another increase between 4 and 8 h. PKC inhibition reduced the initial rise in NO release by 50% and the second increase by 70%. eNOS mRNA and protein levels were also increased in response to shear stress, whereas PKC inhibition prevented this increase. The stimulation of PKC activity with phorbol ester increased eNOS gene expression without increasing NO release. These results suggest that PKC may play different roles in shear stress-mediated release of NO and increased eNOS gene expression. signal transduction; gene regulation; pulmonary blood flow; nitric oxide synthase; protein kinase C