Comet electrophoresis of blood nucleated cells in genotoxicity assessment

Genotoxicity evaluations of several different chemicals including L-4-oxalysine, 10-Hydroxycamptothecin (HCT), 19-norprogesteron (ST1435), dimethyl sulfoxide (Me2SO), bleomycin (BLM), and mitomycin C (MMC). Alkaline comet assay in vitro (single cell gel) (SCG). L-4-oxalysine and HCT did not cause di...

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Veröffentlicht in:	Zhōngguó yàolĭ xuébào 1999-11, Vol.20 (11), p.975
Hauptverfasser:	Guo, J Y, Tu, Z H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bleomycin - toxicity Cell Survival - drug effects Comet Assay Dimethyl Sulfoxide - toxicity DNA Damage - drug effects Lymphocytes - cytology Mitomycin - toxicity Norprogesterones - toxicity Random Allocation Rats Rats, Sprague-Dawley
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Beschreibung
Zusammenfassung:	Genotoxicity evaluations of several different chemicals including L-4-oxalysine, 10-Hydroxycamptothecin (HCT), 19-norprogesteron (ST1435), dimethyl sulfoxide (Me2SO), bleomycin (BLM), and mitomycin C (MMC). Alkaline comet assay in vitro (single cell gel) (SCG). L-4-oxalysine and HCT did not cause directly DNA damage. ST1435, the subdermal implant progestin, had no effect on DNA damage until the dose level up to 4 mmol.L-1. Me2SO did not increase DNA damage at concentration below 2%, but showed a concentration-dependent DNA damage at > or = 4%. Bleomycin and mitomycin C demonstrated a strong dose-dependent DNA damage. Comet assay as a tool to test the genotoxicity of suspected chemicals, is rapid, simple, sensitive, good reproducible, and inexpensive.
ISSN:	0253-9756