An anchored YAC-STS framework for the rat genome

We report here the first YAC-STS framework for the rat genome. A total of 417 anchor microsatellite markers were used to screen a 10-fold redundant YAC library. One or more unambiguous YACs were identified for 372 markers. Assuming the genetic length of the rat genome to be 2,000 cM (Bihoreau et al....

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Veröffentlicht in:	Cytogenetic and genome research 2000-01, Vol.89 (3-4), p.168-170
Hauptverfasser:	Cai, L., Lindpaintner, K., Browne, J., Gruetzner, F., Haaf, T., James, M.R., Bihoreau, M.-T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Chromosome Mapping Chromosomes - genetics Chromosomes, Artificial, Yeast Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Genome In Situ Hybridization, Fluorescence Microsatellite Repeats Rats Sequence Tagged Sites Vertebrata
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Beschreibung
Zusammenfassung:	We report here the first YAC-STS framework for the rat genome. A total of 417 anchor microsatellite markers were used to screen a 10-fold redundant YAC library. One or more unambiguous YACs were identified for 372 markers. Assuming the genetic length of the rat genome to be 2,000 cM (Bihoreau et al. 1997b), the YAC-STS framework will provide, on average, one informative YAC clone every 5.4 cM. A total of 111 anchor markers used in this study were derived from known gene regions. We also demonstrated one of the important and immediate uses of this YAC-STS framework, which is to establish a correlation between the genetic and cytogenetic maps in the rat through FISH analysis.
ISSN:	1424-8581 0301-0171 1424-859X
DOI:	10.1159/000015606