A rapid procedure for the isolation of plasmid DNA from environmental bacteria

The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the...

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Veröffentlicht in:	International microbiology 1999-06, Vol.2 (2), p.115
Hauptverfasser:	Ferrús, M A, Alonso, J L, Amorós, I, Hernández, M, Hernández, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques Centrifugation - methods DNA, Bacterial - isolation & purification Electrophoresis, Agar Gel Escherichia coli - genetics Fresh Water Gels Plasmids - genetics Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Salmonella - genetics Salmonella - isolation & purification Water Microbiology Water Pollution
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Zusammenfassung:	The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environmental Salmonella strains, although it was not suitable for Pseudomonas aeruginosa and enterococci strains. The INSTA-MINI-PREP method is a new alternative procedure to screen plasmid contents of Salmonella and E. coli strains rapidly and easily.
ISSN:	1139-6709