Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy

Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy

The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, alt...

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Veröffentlicht in:Biochemical and biophysical research communications 2000-03, Vol.269 (1), p.76
Hauptverfasser: Kalic, M, Lauterwein, J, Büsselmann, G, Kamp, G
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carps - metabolism
Glutamic Acid - chemistry
Magnetic Resonance Spectroscopy
Male
Methionine - analogs & derivatives
Methionine - chemistry
Proline - chemistry
Protons
Semen - chemistry
Spectrum Analysis - methods
Taurine - chemistry
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Zusammenfassung:The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key proton signals were strongly overlapped.
ISSN:0006-291X
DOI:10.1006/bbrc.2000.2250