Infectious Viral DNA of Murine Leukemia Virus
A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from clos...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1975-12, Vol.72 (12), p.4910-4913 |
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| container_end_page | 4913 |
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| container_issue | 12 |
| container_start_page | 4910 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 72 |
| creator | Smotkin, David Gianni, Alessandro M. Rozenblatt, Shmuel Weinberg, Robert A. |
| description | A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 × 106daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 × 106daltons in mass. |
| doi_str_mv | 10.1073/pnas.72.12.4910 |
| format | Article |
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The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 × 106daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 × 106daltons in mass.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.72.12.4910</identifier><identifier>PMID: 1061080</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>3T3 cells ; Cell Line ; Cell lines ; Cultured cells ; DNA ; DNA, Viral - analysis ; DNA, Viral - physiology ; Infections ; Kinetics ; Molecules ; Moloney murine leukemia virus - analysis ; Moloney murine leukemia virus - pathogenicity ; Nucleic Acid Conformation ; Nucleic acids ; RNA ; Viral DNA ; Virus Replication ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1975-12, Vol.72 (12), p.4910-4913</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-eab2fd4d4468ba8f34b259d404251438bba5bc87e2bd816def0a2f46dd6e7bd63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/72/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/65314$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/65314$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1061080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smotkin, David</creatorcontrib><creatorcontrib>Gianni, Alessandro M.</creatorcontrib><creatorcontrib>Rozenblatt, Shmuel</creatorcontrib><creatorcontrib>Weinberg, Robert A.</creatorcontrib><title>Infectious Viral DNA of Murine Leukemia Virus</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 × 106daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 × 106daltons in mass.</description><subject>3T3 cells</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cultured cells</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - physiology</subject><subject>Infections</subject><subject>Kinetics</subject><subject>Molecules</subject><subject>Moloney murine leukemia virus - analysis</subject><subject>Moloney murine leukemia virus - pathogenicity</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic acids</subject><subject>RNA</subject><subject>Viral DNA</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAURS0EKqUwIyGBsjElfXYcxxkYqvJVqcACrJYd25DSJlWcIPj3OLSUsjB5OOe-53cROsYQYUjj4bKULkpJhElEMww7qI8hwyGjGeyiPgBJQ04J3UcHzs0AIEs49FAPA8PAoY_CSWlN3hRV64Lnopbz4PJ-FFQ2uGvrojTB1LRvZlHIDrbuEO1ZOXfmaP0O0NP11eP4Npw-3EzGo2mY0xia0EhFrKaaUsaV5DamiiSZpkBJgmnMlZKJynlqiNIcM20sSGIp05qZVGkWD9DFau6yVQujc1M2_mtiWRcLWX-KShbiLymLV_FSvYuYc3-uzw9X-byunKuN3UQxiK430fUmUiIwEV1vPnG6vXHL_y7K8_M174I_9HeAsO183piPxptn_5peOFkJM9dU9cZgSey7-QJRr4rQ</recordid><startdate>19751201</startdate><enddate>19751201</enddate><creator>Smotkin, David</creator><creator>Gianni, Alessandro M.</creator><creator>Rozenblatt, Shmuel</creator><creator>Weinberg, Robert A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19751201</creationdate><title>Infectious Viral DNA of Murine Leukemia Virus</title><author>Smotkin, David ; Gianni, Alessandro M. ; Rozenblatt, Shmuel ; Weinberg, Robert A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-eab2fd4d4468ba8f34b259d404251438bba5bc87e2bd816def0a2f46dd6e7bd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>3T3 cells</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cultured cells</topic><topic>DNA</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - physiology</topic><topic>Infections</topic><topic>Kinetics</topic><topic>Molecules</topic><topic>Moloney murine leukemia virus - analysis</topic><topic>Moloney murine leukemia virus - pathogenicity</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic acids</topic><topic>RNA</topic><topic>Viral DNA</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smotkin, David</creatorcontrib><creatorcontrib>Gianni, Alessandro M.</creatorcontrib><creatorcontrib>Rozenblatt, Shmuel</creatorcontrib><creatorcontrib>Weinberg, Robert A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smotkin, David</au><au>Gianni, Alessandro M.</au><au>Rozenblatt, Shmuel</au><au>Weinberg, Robert A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infectious Viral DNA of Murine Leukemia Virus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1975-12-01</date><risdate>1975</risdate><volume>72</volume><issue>12</issue><spage>4910</spage><epage>4913</epage><pages>4910-4913</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 × 106daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 × 106daltons in mass.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1061080</pmid><doi>10.1073/pnas.72.12.4910</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1975-12, Vol.72 (12), p.4910-4913 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_1061080 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 3T3 cells Cell Line Cell lines Cultured cells DNA DNA, Viral - analysis DNA, Viral - physiology Infections Kinetics Molecules Moloney murine leukemia virus - analysis Moloney murine leukemia virus - pathogenicity Nucleic Acid Conformation Nucleic acids RNA Viral DNA Virus Replication Viruses |
| title | Infectious Viral DNA of Murine Leukemia Virus |
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