Infectious Viral DNA of Murine Leukemia Virus

A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from clos...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1975-12, Vol.72 (12), p.4910-4913
Hauptverfasser:	Smotkin, David, Gianni, Alessandro M., Rozenblatt, Shmuel, Weinberg, Robert A.
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 cells Cell Line Cell lines Cultured cells DNA DNA, Viral - analysis DNA, Viral - physiology Infections Kinetics Molecules Moloney murine leukemia virus - analysis Moloney murine leukemia virus - pathogenicity Nucleic Acid Conformation Nucleic acids RNA Viral DNA Virus Replication Viruses
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Beschreibung
Zusammenfassung:	A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 × 106daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 × 106daltons in mass.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.72.12.4910