Instrument Considerations in the Transfer of Chromatographic Methods, Part III: Aligning Performance of Modules

Many laboratories spend a large amount of time and effort transferring chromatographic methods. This is the final installment of a three-part series describing the challenges and solutions commonly associated with this activity. Part I dealt with the method itself. In part II, the characterization of the system was emphasized. In part III, the techniques for bringing the individual modules of the two systems into alignment so that equivalent results are obtained. As expected, the pumping system requires much attention, but the injector, the column temperature control, and the detector must also be carefully managed.