Optimized nutrient additives for fed-batch cultures

Cell density, longevity, and expression are the economics of cell cultures in biopharmaceutical production - and rich growth media are the investment. Glucose and glutamine are primary energy sources for culture growth, but their associated metabolic wastes can actually harm cell cultures. Adapting cell lines to reduced levels of glucose and glutamine, then feeding the cultures chemically defined nutrient supplements, increases cell viability, maximizes cell density, and increases product expression.