Simultaneous Quantitation of Buprenorphine and Its Metabolites Using LC-MS

Simultaneous Quantitation of Buprenorphine and Its Metabolites Using LC-MS

The chromatographic conditions were optimized on an Alltech Alltima HP C18 column using a mobile phase containing acetonitrile and 25 mM ammonium acetate buffer. All of the analytes were eluted in the 3.6-7 min range. The chromatographic gradient was optimized such that there was sufficient time for...

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Veröffentlicht in:Spectroscopy 2016-07, p.15
Hauptverfasser: Joshi, Anand A, Maharao, Neha V, Gerk, Phillip M
Format: Artikel
Sprache:eng
Schlagworte:
Accuracy
Chemical compounds
Chromatography
Mass spectrometry
Precision
Studies
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Zusammenfassung:The chromatographic conditions were optimized on an Alltech Alltima HP C18 column using a mobile phase containing acetonitrile and 25 mM ammonium acetate buffer. All of the analytes were eluted in the 3.6-7 min range. The chromatographic gradient was optimized such that there was sufficient time for the buffer salts to be eluted to the solvent waste (initial 3 min of the run time) followed by a sequential elution of all four analytes including the internal standard until 7 min.
ISSN:0887-6703
1939-1900