Site-Specific Conjugation of ScFvs Antibodies to Nanoparticles by Bioorthogonal Strain-Promoted Alkyne-Nitrone Cycloaddition

Particularly suitable: An N‐terminal serine mutant of anti‐HER2 scFv antibody was conjugated to polymer‐coated magnetofluorescent nanoparticles by strain‐promoted alkyne–nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2‐positive breast ca...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-01, Vol.51 (2), p.496-499
Hauptverfasser:	Colombo, Miriam, Sommaruga, Silvia, Mazzucchelli, Serena, Polito, Laura, Verderio, Paolo, Galeffi, Patrizia, Corsi, Fabio, Tortora, Paolo, Prosperi, Davide
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkynes - chemistry bioorthogonal reaction Cell Line, Tumor cell targeting Cyclization cycloaddition Humans Mutation nanoparticles Nanoparticles - chemistry Nitrogen Oxides - chemistry Receptor, ErbB-2 - analysis Receptor, ErbB-2 - immunology scFv antibody Single-Chain Antibodies - chemistry Single-Chain Antibodies - genetics Single-Chain Antibodies - immunology
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Beschreibung
Zusammenfassung:	Particularly suitable: An N‐terminal serine mutant of anti‐HER2 scFv antibody was conjugated to polymer‐coated magnetofluorescent nanoparticles by strain‐promoted alkyne–nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2‐positive breast cancer cells.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201106775