Phosphopeptide-Dependent Labeling of 14-3-3 ζ Proteins by Fusicoccin-Based Fluorescent Probes

Fluorescent combination: Cell‐penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14–3–3 proteins and phosphopeptide ligands, whereupon the probes site‐specifically attach a fluorescent tag onto the surface of the 14–3–3 proteins.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Angewandte Chemie International Edition 2012-01, Vol.51 (2), p.509-512
Hauptverfasser:	Takahashi, Michiko, Kawamura, Akie, Kato, Nobuo, Nishi, Tsuyoshi, Hamachi, Itaru, Ohkanda, Junko
Format:	Artikel
Sprache:	eng
Schlagworte:	14-3-3 Proteins - analysis 14-3-3 Proteins - chemistry Cell Line Fluorescent Dyes - chemistry fluorescent probes Glycosides - chemistry Humans Models, Molecular natural products phosphopeptides Phosphopeptides - chemistry protein labeling protein-protein interactions Staining and Labeling
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Fluorescent combination: Cell‐penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14–3–3 proteins and phosphopeptide ligands, whereupon the probes site‐specifically attach a fluorescent tag onto the surface of the 14–3–3 proteins.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201106995