Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution
Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid‐state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivi...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2012-01, Vol.51 (2), p.432-435 |
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| container_end_page | 435 |
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| container_issue | 2 |
| container_start_page | 432 |
| container_title | Angewandte Chemie International Edition |
| container_volume | 51 |
| creator | Jacso, Tomas Franks, W. Trent Rose, Honor Fink, Uwe Broecker, Jana Keller, Sandro Oschkinat, Hartmut Reif, Bernd |
| description | Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid‐state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivity to observe the membrane protein of interest. |
| doi_str_mv | 10.1002/anie.201104987 |
| format | Article |
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| ispartof | Angewandte Chemie International Edition, 2012-01, Vol.51 (2), p.432-435 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Cell Membrane - chemistry Escherichia coli - chemistry Escherichia coli Proteins - analysis membrane proteins Membrane Proteins - analysis Models, Molecular NMR spectroscopy Nuclear Magnetic Resonance, Biomolecular - methods structural biology structure elucidation |
| title | Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution |
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