Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution

Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution

Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid‐state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivi...

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Veröffentlicht in:Angewandte Chemie International Edition 2012-01, Vol.51 (2), p.432-435
Hauptverfasser: Jacso, Tomas, Franks, W. Trent, Rose, Honor, Fink, Uwe, Broecker, Jana, Keller, Sandro, Oschkinat, Hartmut, Reif, Bernd
Format: Artikel
Sprache:eng
Schlagworte:
Cell Membrane - chemistry
Escherichia coli - chemistry
Escherichia coli Proteins - analysis
membrane proteins
Membrane Proteins - analysis
Models, Molecular
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
structural biology
structure elucidation
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Zusammenfassung:Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid‐state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivity to observe the membrane protein of interest.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201104987