High community faecal carriage rates of CTX-M ESBL-producing Escherichia coli in a specific population group in Birmingham, UK
To determine the proportion of E. coli carrying specific CTX-M extended-spectrum β-lactamase (ESBL) genotypes in a community population of East and North Birmingham. General practice and outpatient stool samples from 732 individuals submitted for examination for faecal pathogens in 2010 were screene...
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Veröffentlicht in: | Journal of antimicrobial chemotherapy 2012-05, Vol.67 (5), p.1108-1113 |
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creator | WICKRAMASINGHE, Nimal H LI XU EUSTACE, Andrew SHABIR, Sahida SALUJA, Tranprit HAWKEY, Peter M |
description | To determine the proportion of E. coli carrying specific CTX-M extended-spectrum β-lactamase (ESBL) genotypes in a community population of East and North Birmingham.
General practice and outpatient stool samples from 732 individuals submitted for examination for faecal pathogens in 2010 were screened for ESBL-producing E. coli using chromogenic agar. Multiplex PCR, denaturing HPLC, DNA sequencing and PFGE were used to determine the CTX-M genotype and clonal subtype. Isolates from people were assigned to 'Europe', 'Middle East/South Asia' (MESA) or 'uncategorized' groups using software to determine probable global origin based on the subject's full name.
Prevalence of CTX-M carriage in the sample population was 11.3%. There was a statistically significant difference (P < 0.001) between carriage in the Europe group (8.1%) and the MESA group (22.8%). There was also a higher rate of carriage of CTX-M-15-producing E. coli (P < 0.001) in MESA subjects.
The high community carriage rate and the significant difference in carriage between the Europe and MESA subjects may have important consequences for therapy. If the rising trend in carriage of bacteria producing ESBLs continues, guidelines for empirical therapy for patients presenting from the community may need to be modified. The findings also raise the concern that the pattern and routes of spread of CTX-M-15 may be replicated in the future by broader-spectrum β-lactamases, such as New Delhi metallo-β-lactamase ('NDM-1'). |
doi_str_mv | 10.1093/jac/dks018 |
format | Article |
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General practice and outpatient stool samples from 732 individuals submitted for examination for faecal pathogens in 2010 were screened for ESBL-producing E. coli using chromogenic agar. Multiplex PCR, denaturing HPLC, DNA sequencing and PFGE were used to determine the CTX-M genotype and clonal subtype. Isolates from people were assigned to 'Europe', 'Middle East/South Asia' (MESA) or 'uncategorized' groups using software to determine probable global origin based on the subject's full name.
Prevalence of CTX-M carriage in the sample population was 11.3%. There was a statistically significant difference (P < 0.001) between carriage in the Europe group (8.1%) and the MESA group (22.8%). There was also a higher rate of carriage of CTX-M-15-producing E. coli (P < 0.001) in MESA subjects.
The high community carriage rate and the significant difference in carriage between the Europe and MESA subjects may have important consequences for therapy. If the rising trend in carriage of bacteria producing ESBLs continues, guidelines for empirical therapy for patients presenting from the community may need to be modified. The findings also raise the concern that the pattern and routes of spread of CTX-M-15 may be replicated in the future by broader-spectrum β-lactamases, such as New Delhi metallo-β-lactamase ('NDM-1').</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dks018</identifier><identifier>PMID: 22403261</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Agar ; Ambulatory Care ; Animal populations ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobial agents ; Bacteriological Techniques ; beta -Lactamase ; beta-Lactamases - secretion ; Biological and medical sciences ; Carrier State - epidemiology ; Carrier State - microbiology ; Chromatography, High Pressure Liquid ; Cluster Analysis ; Computer programs ; DNA sequencing ; E coli ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - isolation & purification ; Escherichia coli Infections - classification ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - genetics ; Escherichia coli Infections - microbiology ; Feces ; Feces - microbiology ; General Practice ; Genotype ; Genotypes ; High-performance liquid chromatography ; Humans ; Medical sciences ; Metallo- beta -lactamase ; Pathogens ; Pharmacology. Drug treatments ; Polymerase chain reaction ; Population Groups ; Prevalence ; Sequence Analysis, DNA ; software ; Statistical analysis ; United Kingdom - epidemiology</subject><ispartof>Journal of antimicrobial chemotherapy, 2012-05, Vol.67 (5), p.1108-1113</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright Oxford Publishing Limited(England) May 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-14ea2ab18932a8483c4bf7489e5a094564c7d64db3cd337f75c7be590b31ad943</citedby><cites>FETCH-LOGICAL-c413t-14ea2ab18932a8483c4bf7489e5a094564c7d64db3cd337f75c7be590b31ad943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25784098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22403261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WICKRAMASINGHE, Nimal H</creatorcontrib><creatorcontrib>LI XU</creatorcontrib><creatorcontrib>EUSTACE, Andrew</creatorcontrib><creatorcontrib>SHABIR, Sahida</creatorcontrib><creatorcontrib>SALUJA, Tranprit</creatorcontrib><creatorcontrib>HAWKEY, Peter M</creatorcontrib><title>High community faecal carriage rates of CTX-M ESBL-producing Escherichia coli in a specific population group in Birmingham, UK</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>To determine the proportion of E. coli carrying specific CTX-M extended-spectrum β-lactamase (ESBL) genotypes in a community population of East and North Birmingham.
General practice and outpatient stool samples from 732 individuals submitted for examination for faecal pathogens in 2010 were screened for ESBL-producing E. coli using chromogenic agar. Multiplex PCR, denaturing HPLC, DNA sequencing and PFGE were used to determine the CTX-M genotype and clonal subtype. Isolates from people were assigned to 'Europe', 'Middle East/South Asia' (MESA) or 'uncategorized' groups using software to determine probable global origin based on the subject's full name.
Prevalence of CTX-M carriage in the sample population was 11.3%. There was a statistically significant difference (P < 0.001) between carriage in the Europe group (8.1%) and the MESA group (22.8%). There was also a higher rate of carriage of CTX-M-15-producing E. coli (P < 0.001) in MESA subjects.
The high community carriage rate and the significant difference in carriage between the Europe and MESA subjects may have important consequences for therapy. If the rising trend in carriage of bacteria producing ESBLs continues, guidelines for empirical therapy for patients presenting from the community may need to be modified. The findings also raise the concern that the pattern and routes of spread of CTX-M-15 may be replicated in the future by broader-spectrum β-lactamases, such as New Delhi metallo-β-lactamase ('NDM-1').</description><subject>Agar</subject><subject>Ambulatory Care</subject><subject>Animal populations</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobial agents</subject><subject>Bacteriological Techniques</subject><subject>beta -Lactamase</subject><subject>beta-Lactamases - secretion</subject><subject>Biological and medical sciences</subject><subject>Carrier State - epidemiology</subject><subject>Carrier State - microbiology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cluster Analysis</subject><subject>Computer programs</subject><subject>DNA sequencing</subject><subject>E coli</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - classification</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - genetics</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>General Practice</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>High-performance liquid chromatography</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Metallo- beta -lactamase</subject><subject>Pathogens</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase chain reaction</subject><subject>Population Groups</subject><subject>Prevalence</subject><subject>Sequence Analysis, DNA</subject><subject>software</subject><subject>Statistical analysis</subject><subject>United Kingdom - epidemiology</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c1u1DAUBWALgei0sOEBkCWEQKihdvy_pKMprRjEglZiF904zoyHJA52suimz45HMwWJBSsv7nePrnUQekXJR0oMu9iBvWh-JkL1E7SgXJKiJIY-RQvCiCgUF-wEnaa0I4RIIfVzdFKWnLBS0gV6uPabLbah7-fBT_e4BWehwxZi9LBxOMLkEg4tXt7-KL7i1ffLdTHG0MzWDxu8SnbrordbDzmj89gPGHAanfWtt3gM49zB5MOANzHM43586WOfV7fQn-O7Ly_Qsxa65F4e3zN0d7W6XV4X62-fb5af1oXllE0F5Q5KqKk2rATNNbO8bhXXxgkghgvJrWokb2pmG8ZUq4RVtROG1IxCYzg7Q-8Oufn2X7NLU9X7ZF3XweDCnCpjmCFa8jLL9_-VlFBlFJeKZPrmH7oLcxzyP_aKU8m5UFl9OCgbQ0rRtdUYfQ_xPqNq31-V-6sO_WX8-hg5171r_tDHwjJ4ewSQclFthMH69NcJpTkxmv0GYIWiHw</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>WICKRAMASINGHE, Nimal H</creator><creator>LI XU</creator><creator>EUSTACE, Andrew</creator><creator>SHABIR, Sahida</creator><creator>SALUJA, Tranprit</creator><creator>HAWKEY, Peter M</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>High community faecal carriage rates of CTX-M ESBL-producing Escherichia coli in a specific population group in Birmingham, UK</title><author>WICKRAMASINGHE, Nimal H ; LI XU ; EUSTACE, Andrew ; SHABIR, Sahida ; SALUJA, Tranprit ; HAWKEY, Peter M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-14ea2ab18932a8483c4bf7489e5a094564c7d64db3cd337f75c7be590b31ad943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agar</topic><topic>Ambulatory Care</topic><topic>Animal populations</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antimicrobial agents</topic><topic>Bacteriological Techniques</topic><topic>beta -Lactamase</topic><topic>beta-Lactamases - secretion</topic><topic>Biological and medical sciences</topic><topic>Carrier State - epidemiology</topic><topic>Carrier State - microbiology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cluster Analysis</topic><topic>Computer programs</topic><topic>DNA sequencing</topic><topic>E coli</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Infections - classification</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - genetics</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>General Practice</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>High-performance liquid chromatography</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Metallo- beta -lactamase</topic><topic>Pathogens</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymerase chain reaction</topic><topic>Population Groups</topic><topic>Prevalence</topic><topic>Sequence Analysis, DNA</topic><topic>software</topic><topic>Statistical analysis</topic><topic>United Kingdom - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WICKRAMASINGHE, Nimal H</creatorcontrib><creatorcontrib>LI XU</creatorcontrib><creatorcontrib>EUSTACE, Andrew</creatorcontrib><creatorcontrib>SHABIR, Sahida</creatorcontrib><creatorcontrib>SALUJA, Tranprit</creatorcontrib><creatorcontrib>HAWKEY, Peter M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WICKRAMASINGHE, Nimal H</au><au>LI XU</au><au>EUSTACE, Andrew</au><au>SHABIR, Sahida</au><au>SALUJA, Tranprit</au><au>HAWKEY, Peter M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High community faecal carriage rates of CTX-M ESBL-producing Escherichia coli in a specific population group in Birmingham, UK</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>67</volume><issue>5</issue><spage>1108</spage><epage>1113</epage><pages>1108-1113</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>To determine the proportion of E. coli carrying specific CTX-M extended-spectrum β-lactamase (ESBL) genotypes in a community population of East and North Birmingham.
General practice and outpatient stool samples from 732 individuals submitted for examination for faecal pathogens in 2010 were screened for ESBL-producing E. coli using chromogenic agar. Multiplex PCR, denaturing HPLC, DNA sequencing and PFGE were used to determine the CTX-M genotype and clonal subtype. Isolates from people were assigned to 'Europe', 'Middle East/South Asia' (MESA) or 'uncategorized' groups using software to determine probable global origin based on the subject's full name.
Prevalence of CTX-M carriage in the sample population was 11.3%. There was a statistically significant difference (P < 0.001) between carriage in the Europe group (8.1%) and the MESA group (22.8%). There was also a higher rate of carriage of CTX-M-15-producing E. coli (P < 0.001) in MESA subjects.
The high community carriage rate and the significant difference in carriage between the Europe and MESA subjects may have important consequences for therapy. If the rising trend in carriage of bacteria producing ESBLs continues, guidelines for empirical therapy for patients presenting from the community may need to be modified. The findings also raise the concern that the pattern and routes of spread of CTX-M-15 may be replicated in the future by broader-spectrum β-lactamases, such as New Delhi metallo-β-lactamase ('NDM-1').</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>22403261</pmid><doi>10.1093/jac/dks018</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
subjects | Agar Ambulatory Care Animal populations Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobial agents Bacteriological Techniques beta -Lactamase beta-Lactamases - secretion Biological and medical sciences Carrier State - epidemiology Carrier State - microbiology Chromatography, High Pressure Liquid Cluster Analysis Computer programs DNA sequencing E coli Electrophoresis, Gel, Pulsed-Field Escherichia coli Escherichia coli - enzymology Escherichia coli - isolation & purification Escherichia coli Infections - classification Escherichia coli Infections - epidemiology Escherichia coli Infections - genetics Escherichia coli Infections - microbiology Feces Feces - microbiology General Practice Genotype Genotypes High-performance liquid chromatography Humans Medical sciences Metallo- beta -lactamase Pathogens Pharmacology. Drug treatments Polymerase chain reaction Population Groups Prevalence Sequence Analysis, DNA software Statistical analysis United Kingdom - epidemiology |
title | High community faecal carriage rates of CTX-M ESBL-producing Escherichia coli in a specific population group in Birmingham, UK |
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