Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells

We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along mic...

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Veröffentlicht in:	Lab on a chip 2012-05, Vol.12 (9), p.1591-1593
Hauptverfasser:	Oyama, Kotaro, Takabayashi, Masao, Takei, Yoshiaki, Arai, Satoshi, Takeoka, Shinji, Ishiwata, Shin'ichi, Suzuki, Madoka
Format:	Artikel
Sprache:	eng
Schlagworte:	Endosomes - chemistry Fluorescein-5-isothiocyanate Fluorescent Dyes - chemistry HeLa Cells Humans Hydrogen-Ion Concentration Lysosomes - chemistry Microscopy, Confocal Nanoparticles - chemistry Nanotechnology - instrumentation Osmolar Concentration Single-Cell Analysis - instrumentation Single-Cell Analysis - methods Thermometers
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Beschreibung
Zusammenfassung:	We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4-10) and ionic strength (0-500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers".
ISSN:	1473-0197 1473-0189
DOI:	10.1039/c2lc00014h