Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by bulked segregant analysis

In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F4 lines were distributed into two pools, each consisting of the most resistant and of...

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Veröffentlicht in:	Theoretical and applied genetics 1997-09, Vol.95 (4), p.671-677
Hauptverfasser:	Chague, V, Mercier, J.C, Guenard, M, Courcel, A. de, Vedel, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences chromosome mapping Classical genetics, quantitative genetics, hybrids Development and progression disease resistance Diseases and pests Fundamental and applied biological sciences. Psychology Genetic aspects Genetic markers genetic resistance genetic techniques and protocols Genetics Genetics of eukaryotes. Biological and molecular evolution Genomics loci Lycopersicon esculentum Physiological aspects Plant diseases Plant genetics Plant immunology Pteridophyta, spermatophyta Quantitative genetics quantitative traits random amplified polymorphic DNA technique Solanum lycopersicum var. lycopersicum Solanum pimpinellifolium Tomato yellow leaf curl virus Tomatoes Vegetals
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Beschreibung
Zusammenfassung:	In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F4 lines were distributed into two pools, each consisting of the most resistant and of the most susceptible individuals, respectively. Both pools were screened using 600 random primers. Four RAPD markers were found to be linked to a QTL responsible for up to 27.7% of the resistance. These markers, localized in the same linkage group within a distance of 17.3 cM, were mapped to chromosome 6 on the tomato RFLP map.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s001220050611