Light sheet microscopy of living or cleared specimens
Highlights ► We review the history of light sheet microscopy and discuss recent technical breakthroughs. ► We discuss tissue clearing methods and their combination with light sheet imaging. ► Light sheet microscopy provides exceptionally high imaging speeds and low rates of photo-bleaching. ► Recent...
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| Veröffentlicht in: | Current opinion in neurobiology 2012-02, Vol.22 (1), p.138-143 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | Highlights ► We review the history of light sheet microscopy and discuss recent technical breakthroughs. ► We discuss tissue clearing methods and their combination with light sheet imaging. ► Light sheet microscopy provides exceptionally high imaging speeds and low rates of photo-bleaching. ► Recent technical advances include improvements in spatial resolution and penetration depth. ► Light sheet microscopy is a powerful emerging tool for the observation of live and fixed specimens. |
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| ISSN: | 0959-4388 1873-6882 |
| DOI: | 10.1016/j.conb.2011.08.003 |