Vaccination with coxsackievirus B3 virus-like particles elicits humoral immune response and protects mice against myocarditis

Highlights ► CVB3 capsid proteins expressed in insect cells were able to assemble into VLP. ► The CVB3 VLP was non-infectious in tissue culture. ► SWR (H-2q ) mice vaccinated with CVB3 VLP developed antibodies to CVB3 capsid proteins. ► Vaccinated animals were protected from myocarditis when challen...

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Veröffentlicht in:	Vaccine 2012-03, Vol.30 (13), p.2301-2308
Hauptverfasser:	Zhang, L, Parham, N.J, Zhang, F, Aasa-Chapman, M, Gould, E.A, Zhang, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies Antibodies, Viral - blood antigens Baculoviridae Baculoviridae - genetics Baculoviridae - metabolism Baculovirus Baculovirus expression system Capsid protein Capsid Proteins - immunology Capsid Proteins - metabolism cardiomyopathy Cells, Cultured coat proteins Coxsackievirus B3 Coxsackievirus Infections - immunology Coxsackievirus Infections - prevention & control Coxsackievirus Infections - virology Deoxyribonucleic acid Dilated cardiomyopathy DNA Electron microscopy Enterovirus B, Human - immunology Expression vectors fluorescent antibody technique Genomes Heart diseases Histocompatibility antigen H-2 humoral immunity Immune response Immune response (humoral) Immune system Immunity, Humoral Immunofluorescence Infection Infections Insect cells insect proteins insects Male Mice Myocarditis Myocarditis - immunology Myocarditis - prevention & control Myocarditis - virology pathogenesis Protected animals protein synthesis Proteins Rodents Spodoptera - virology Studies subunit vaccines Sucrose Tissue culture Ultracentrifugation Vaccination Vaccine Vaccines Viral Vaccines - administration & dosage Viral Vaccines - immunology Virion - immunology Virus-like particles viruses Western blotting
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Beschreibung
Zusammenfassung:	Highlights ► CVB3 capsid proteins expressed in insect cells were able to assemble into VLP. ► The CVB3 VLP was non-infectious in tissue culture. ► SWR (H-2q ) mice vaccinated with CVB3 VLP developed antibodies to CVB3 capsid proteins. ► Vaccinated animals were protected from myocarditis when challenged with wild-type CVB3. ► VLP produced from the complete CVB3 coding region gave optimal immune response and protection.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2012.01.061