Organophosphorus acid anhydrolase bio-template for the synthesis of CdS quantum dots

Organophosphorus acid anhydrolase bio-template for the synthesis of CdS quantum dots

A direct conjugation of organophosphorus acid anhydrolase (OPAA) with CdS quantum dots was prepared via arrested precipitation within the enzyme matrix. The bio-conjugate was found not only to retain enzyme conformational structure but also to retain enzyme activity and be effective at detecting dii...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (25), p.7242-7244
Hauptverfasser: Zhao, Liang, Gattás-Asfura, Kerim M, Xu, Jianmin, Patel, Ravi A, Dadlani, Anup, Sillero-Mahinay, Myrna, Cushmore, Marie, Rastogi, Vipin K, Shah, Saumil S, Leblanc, Roger M
Format: Artikel
Sprache:eng
Schlagworte:
Aryldialkylphosphatase - metabolism
Cadmium Compounds - chemical synthesis
Cadmium Compounds - chemistry
Cadmium sulfides
Conjugation
Enzyme activity
Enzymes
Precipitation
Quantum Dots
Spectrum Analysis
Sulfides - chemical synthesis
Sulfides - chemistry
Synthesis
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Zusammenfassung:A direct conjugation of organophosphorus acid anhydrolase (OPAA) with CdS quantum dots was prepared via arrested precipitation within the enzyme matrix. The bio-conjugate was found not only to retain enzyme conformational structure but also to retain enzyme activity and be effective at detecting diisopropyl fluorophosphate (DFP) at the micro molar level.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc10714c