Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice
In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25–100mg/kg) stimulated the IgM and IgG titre expre...
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creator | Abid, Sheikh Khajuria, Anamika Parvaiz, Qazi Sidiq, Tabasum Bhatia, Aruna Singh, Surjeet Ahmad, Shabir Randhawa, M.K. Satti, N.K. Dutt, Prabhu |
description | In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25–100mg/kg) stimulated the IgM and IgG titre expressed in the form of hemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-4, and TNF-α) in serum with respect to T cell interactions, i.e. the proliferation of lymphocytes were significantly increased at 50mg/kg compared with the control. The results in these studies demonstrated the immunostimulatory effect of PNRS-EtOAC in a dose-dependent manner with respect to the macrophage activation possibly expressing the phagocytosis and nitrite production by the enhancement of TNF-α production as a mode of action.
► Immunostimulatory potential of PNRS-EtOAC fraction (Prunus cerasus) investigated. ► Augmentation of HA titre and DTH response to sheep red blood cells (SRBC) revealed. ► A significant enhancement of Th1 (IFN-γ and TNF-α) and Th2 (IL-4) immune response. ► Significant enhancement in CD4, CD8, CD80 and CD86 cell populations revealed. ► Important role of PNRS-EtOAC in the modulation of the immune response highlighted. |
doi_str_mv | 10.1016/j.intimp.2012.02.001 |
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► Immunostimulatory potential of PNRS-EtOAC fraction (Prunus cerasus) investigated. ► Augmentation of HA titre and DTH response to sheep red blood cells (SRBC) revealed. ► A significant enhancement of Th1 (IFN-γ and TNF-α) and Th2 (IL-4) immune response. ► Significant enhancement in CD4, CD8, CD80 and CD86 cell populations revealed. ► Important role of PNRS-EtOAC in the modulation of the immune response highlighted.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2012.02.001</identifier><identifier>PMID: 22366406</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Allergic diseases ; Animals ; B-Lymphocytes - cytology ; B-Lymphocytes - drug effects ; B-Lymphocytes - immunology ; B7-1 Antigen - immunology ; B7-2 Antigen - immunology ; Biological and medical sciences ; Cell Proliferation - drug effects ; Cytokines ; Cytokines - immunology ; Delayed type hypersensitivity reaction ; Erythrocytes - immunology ; Ethanol - chemistry ; Fruit - chemistry ; Hemagglutination - drug effects ; Hypersensitivity, Delayed - immunology ; Immunologic Factors - pharmacology ; Immunopathology ; Macrophages ; Macrophages - drug effects ; Macrophages - immunology ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Nitric oxide ; Nitric Oxide - immunology ; Phagocytosis ; Phagocytosis - drug effects ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; Prunus ; Prunus cerasus ; Sheep ; Skin allergic diseases. Stinging insect allergies ; Solvents - chemistry ; Spleen - cytology ; T-Lymphocytes - cytology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology</subject><ispartof>International immunopharmacology, 2012-04, Vol.12 (4), p.626-634</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-3f5affe04f802d81c7888abb8dd4ca02b4e9f422ca2914b9b2f5e2c905dfa5583</citedby><cites>FETCH-LOGICAL-c391t-3f5affe04f802d81c7888abb8dd4ca02b4e9f422ca2914b9b2f5e2c905dfa5583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567576912000392$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25795101$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22366406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abid, Sheikh</creatorcontrib><creatorcontrib>Khajuria, Anamika</creatorcontrib><creatorcontrib>Parvaiz, Qazi</creatorcontrib><creatorcontrib>Sidiq, Tabasum</creatorcontrib><creatorcontrib>Bhatia, Aruna</creatorcontrib><creatorcontrib>Singh, Surjeet</creatorcontrib><creatorcontrib>Ahmad, Shabir</creatorcontrib><creatorcontrib>Randhawa, M.K.</creatorcontrib><creatorcontrib>Satti, N.K.</creatorcontrib><creatorcontrib>Dutt, Prabhu</creatorcontrib><title>Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25–100mg/kg) stimulated the IgM and IgG titre expressed in the form of hemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-4, and TNF-α) in serum with respect to T cell interactions, i.e. the proliferation of lymphocytes were significantly increased at 50mg/kg compared with the control. The results in these studies demonstrated the immunostimulatory effect of PNRS-EtOAC in a dose-dependent manner with respect to the macrophage activation possibly expressing the phagocytosis and nitrite production by the enhancement of TNF-α production as a mode of action.
► Immunostimulatory potential of PNRS-EtOAC fraction (Prunus cerasus) investigated. ► Augmentation of HA titre and DTH response to sheep red blood cells (SRBC) revealed. ► A significant enhancement of Th1 (IFN-γ and TNF-α) and Th2 (IL-4) immune response. ► Significant enhancement in CD4, CD8, CD80 and CD86 cell populations revealed. ► Important role of PNRS-EtOAC in the modulation of the immune response highlighted.</description><subject>Allergic diseases</subject><subject>Animals</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - immunology</subject><subject>B7-1 Antigen - immunology</subject><subject>B7-2 Antigen - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Delayed type hypersensitivity reaction</subject><subject>Erythrocytes - immunology</subject><subject>Ethanol - chemistry</subject><subject>Fruit - chemistry</subject><subject>Hemagglutination - drug effects</subject><subject>Hypersensitivity, Delayed - immunology</subject><subject>Immunologic Factors - pharmacology</subject><subject>Immunopathology</subject><subject>Macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - immunology</subject><subject>Phagocytosis</subject><subject>Phagocytosis - drug effects</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>Prunus</subject><subject>Prunus cerasus</subject><subject>Sheep</subject><subject>Skin allergic diseases. Stinging insect allergies</subject><subject>Solvents - chemistry</subject><subject>Spleen - cytology</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpaT7af1CKLiUnbyTZsuVLIQlpGlhIDu1ZlaUR1WJZW30E8u8jd7ftrTAwL-KZGfEg9IGSDSW0v9xt3JKd328YoWxDahH6Cp1SMYiGDoS_rpn3Q8OHfjxBZyntKjCQjr5FJ4y1fd-R_hT9uPe-LMEHU2aVQ3zGKRfjIOFgscKTC0pn9wTYxjWEpYbgcf65vhSXV-wxlqUkrCGqVLtb8PXV9vpSY-80vENvrJoTvD_2c_T9y-23m6_N9uHu_uZq2-h2pLlpLVfWAumsIMwIqgchhJomYUynFWFTB6PtGNOKjbSbxolZDkyPhBurOBftObo47N3H8KtAytK7pGGe1QKhJDn2rWCibftKdgdSx5BSBCv30XkVnyUlclUrd_KgVq5qJalFaB37eDxQJg_m79AflxX4dARU0mquwhbt0j-ODyOnvxd9PnBQdTw5iDJpB4sG4yLoLE1w___JCybImgM</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Abid, Sheikh</creator><creator>Khajuria, Anamika</creator><creator>Parvaiz, Qazi</creator><creator>Sidiq, Tabasum</creator><creator>Bhatia, Aruna</creator><creator>Singh, Surjeet</creator><creator>Ahmad, Shabir</creator><creator>Randhawa, M.K.</creator><creator>Satti, N.K.</creator><creator>Dutt, Prabhu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice</title><author>Abid, Sheikh ; Khajuria, Anamika ; Parvaiz, Qazi ; Sidiq, Tabasum ; Bhatia, Aruna ; Singh, Surjeet ; Ahmad, Shabir ; Randhawa, M.K. ; Satti, N.K. ; Dutt, Prabhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-3f5affe04f802d81c7888abb8dd4ca02b4e9f422ca2914b9b2f5e2c905dfa5583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Allergic diseases</topic><topic>Animals</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - immunology</topic><topic>B7-1 Antigen - immunology</topic><topic>B7-2 Antigen - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Delayed type hypersensitivity reaction</topic><topic>Erythrocytes - immunology</topic><topic>Ethanol - chemistry</topic><topic>Fruit - chemistry</topic><topic>Hemagglutination - drug effects</topic><topic>Hypersensitivity, Delayed - immunology</topic><topic>Immunologic Factors - pharmacology</topic><topic>Immunopathology</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - immunology</topic><topic>Phagocytosis</topic><topic>Phagocytosis - drug effects</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>Prunus</topic><topic>Prunus cerasus</topic><topic>Sheep</topic><topic>Skin allergic diseases. Stinging insect allergies</topic><topic>Solvents - chemistry</topic><topic>Spleen - cytology</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abid, Sheikh</creatorcontrib><creatorcontrib>Khajuria, Anamika</creatorcontrib><creatorcontrib>Parvaiz, Qazi</creatorcontrib><creatorcontrib>Sidiq, Tabasum</creatorcontrib><creatorcontrib>Bhatia, Aruna</creatorcontrib><creatorcontrib>Singh, Surjeet</creatorcontrib><creatorcontrib>Ahmad, Shabir</creatorcontrib><creatorcontrib>Randhawa, M.K.</creatorcontrib><creatorcontrib>Satti, N.K.</creatorcontrib><creatorcontrib>Dutt, Prabhu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abid, Sheikh</au><au>Khajuria, Anamika</au><au>Parvaiz, Qazi</au><au>Sidiq, Tabasum</au><au>Bhatia, Aruna</au><au>Singh, Surjeet</au><au>Ahmad, Shabir</au><au>Randhawa, M.K.</au><au>Satti, N.K.</au><au>Dutt, Prabhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>12</volume><issue>4</issue><spage>626</spage><epage>634</epage><pages>626-634</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>In order to evaluate the role of ethyl acetate fraction (PNRS-EtOAC) obtained from the Prunus cerasus fruit in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PNRS-EtOAC (25–100mg/kg) stimulated the IgM and IgG titre expressed in the form of hemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-4, and TNF-α) in serum with respect to T cell interactions, i.e. the proliferation of lymphocytes were significantly increased at 50mg/kg compared with the control. The results in these studies demonstrated the immunostimulatory effect of PNRS-EtOAC in a dose-dependent manner with respect to the macrophage activation possibly expressing the phagocytosis and nitrite production by the enhancement of TNF-α production as a mode of action.
► Immunostimulatory potential of PNRS-EtOAC fraction (Prunus cerasus) investigated. ► Augmentation of HA titre and DTH response to sheep red blood cells (SRBC) revealed. ► A significant enhancement of Th1 (IFN-γ and TNF-α) and Th2 (IL-4) immune response. ► Significant enhancement in CD4, CD8, CD80 and CD86 cell populations revealed. ► Important role of PNRS-EtOAC in the modulation of the immune response highlighted.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>22366406</pmid><doi>10.1016/j.intimp.2012.02.001</doi><tpages>9</tpages></addata></record> |
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subjects | Allergic diseases Animals B-Lymphocytes - cytology B-Lymphocytes - drug effects B-Lymphocytes - immunology B7-1 Antigen - immunology B7-2 Antigen - immunology Biological and medical sciences Cell Proliferation - drug effects Cytokines Cytokines - immunology Delayed type hypersensitivity reaction Erythrocytes - immunology Ethanol - chemistry Fruit - chemistry Hemagglutination - drug effects Hypersensitivity, Delayed - immunology Immunologic Factors - pharmacology Immunopathology Macrophages Macrophages - drug effects Macrophages - immunology Male Medical sciences Mice Mice, Inbred BALB C Nitric oxide Nitric Oxide - immunology Phagocytosis Phagocytosis - drug effects Pharmacology. Drug treatments Plant Extracts - pharmacology Prunus Prunus cerasus Sheep Skin allergic diseases. Stinging insect allergies Solvents - chemistry Spleen - cytology T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - immunology |
title | Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice |
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