Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley
A doubled haploid (DH) barley ( Hordeum vulgare L.) population of 334 lines (ND24260 × Flagship) genotyped with DArT markers was used to map genes for adult plant resistance (APR) to leaf rust ( Puccinia hordei Otth) under field conditions in Australia and Uruguay. The Australian barley cultivar Fla...
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Veröffentlicht in: | Theoretical and applied genetics 2011-06, Vol.123 (1), p.55-68 |
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Zusammenfassung: | A doubled haploid (DH) barley (
Hordeum vulgare
L.) population of 334 lines (ND24260 × Flagship) genotyped with DArT markers was used to map genes for adult plant resistance (APR) to leaf rust (
Puccinia hordei
Otth) under field conditions in Australia and Uruguay. The Australian barley cultivar Flagship carries an APR gene (
qRphFlag
) derived from the cultivar Vada. Association analysis and composite interval mapping identified two genes conferring APR in this DH population.
qRphFlag
was mapped to the short arm of chromosome 5H (5HS), accounting for 64–85% of the phenotypic variation across four field environments and 56% under controlled environmental conditions (CEC). A second quantitative trait locus (QTL) from ND24260 (
qRphND
) with smaller effect was mapped to chromosome 6HL. In the absence of
qRphFlag
,
qRphND
conferred only a low level of resistance. DH lines displaying the highest level of APR carried both genes. Sequence information for the critical DArT marker bPb-0837 (positioned at 21.2 cM on chromosome 5HS) was used to develop
bPb
-
0837
-
PCR
, a simple PCR-based marker for
qRphFlag
. The 245 bp fragment for
bPb
-
0837
-
PCR
was detected in a range of barley cultivars known to possess APR, which was consistent with previous tests of allelism, demonstrating that the
qRphFlag
resistant allele is common in leaf rust resistant cultivars derived from Vada and Emir.
qRphFlag
has been designated
Rph20
, the first gene conferring APR to
P. hordei
to be characterised in barley. The PCR marker will likely be effective in marker-assisted selection for
Rph20
. |
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ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-011-1566-z |