Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species
A 4.1 kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta . A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. Th...
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| Veröffentlicht in: | European journal of plant pathology 2011-10, Vol.131 (2), p.261-268 |
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| creator | Moleleki, Ntsane Wingfield, Michael J. Wingfield, Brenda D. Preisig, Oliver |
| description | A 4.1 kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen
Diaporthe perjuncta
. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different
Diaporthe
spp. The transfected species included three
D. ambigua
isolates and an unidentified
Phomopsis
asexual state of a
Diaporthe
sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DRV1 did not reduce growth rate in two of the three
D. ambigua
or the
Phomopsis
sp. significantly. Pathogenicity studies showed that the transfected isolates have reduced aggresiveness. |
| doi_str_mv | 10.1007/s10658-011-9804-4 |
| format | Article |
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Diaporthe perjuncta
. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different
Diaporthe
spp. The transfected species included three
D. ambigua
isolates and an unidentified
Phomopsis
asexual state of a
Diaporthe
sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DRV1 did not reduce growth rate in two of the three
D. ambigua
or the
Phomopsis
sp. significantly. Pathogenicity studies showed that the transfected isolates have reduced aggresiveness.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-011-9804-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Biological and medical sciences ; Biomedical and Life Sciences ; Diaporthe ; Ecology ; Fundamental and applied biological sciences. Psychology ; Genomes ; Life Sciences ; Pathogens ; Phytopathology. Animal pests. Plant and forest protection ; Plant Pathology ; Plant Sciences ; Ribonucleic acid ; RNA ; Viruses</subject><ispartof>European journal of plant pathology, 2011-10, Vol.131 (2), p.261-268</ispartof><rights>KNPV 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-de5cbe55385e2d0170a733daa3ba05e696718e3191a3b2ec304d168de76615ad3</citedby><cites>FETCH-LOGICAL-c377t-de5cbe55385e2d0170a733daa3ba05e696718e3191a3b2ec304d168de76615ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-011-9804-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-011-9804-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24487306$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Moleleki, Ntsane</creatorcontrib><creatorcontrib>Wingfield, Michael J.</creatorcontrib><creatorcontrib>Wingfield, Brenda D.</creatorcontrib><creatorcontrib>Preisig, Oliver</creatorcontrib><title>Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>A 4.1 kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen
Diaporthe perjuncta
. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different
Diaporthe
spp. The transfected species included three
D. ambigua
isolates and an unidentified
Phomopsis
asexual state of a
Diaporthe
sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DRV1 did not reduce growth rate in two of the three
D. ambigua
or the
Phomopsis
sp. significantly. Pathogenicity studies showed that the transfected isolates have reduced aggresiveness.</description><subject>Agriculture</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Diaporthe</subject><subject>Ecology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genomes</subject><subject>Life Sciences</subject><subject>Pathogens</subject><subject>Phytopathology. Animal pests. 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Psychology</topic><topic>Genomes</topic><topic>Life Sciences</topic><topic>Pathogens</topic><topic>Phytopathology. Animal pests. 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Diaporthe perjuncta
. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different
Diaporthe
spp. The transfected species included three
D. ambigua
isolates and an unidentified
Phomopsis
asexual state of a
Diaporthe
sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DRV1 did not reduce growth rate in two of the three
D. ambigua
or the
Phomopsis
sp. significantly. Pathogenicity studies showed that the transfected isolates have reduced aggresiveness.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-011-9804-4</doi><tpages>8</tpages></addata></record> |
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| subjects | Agriculture Biological and medical sciences Biomedical and Life Sciences Diaporthe Ecology Fundamental and applied biological sciences. Psychology Genomes Life Sciences Pathogens Phytopathology. Animal pests. Plant and forest protection Plant Pathology Plant Sciences Ribonucleic acid RNA Viruses |
| title | Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species |
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