Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species
A 4.1 kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta . A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. Th...
Gespeichert in:
Veröffentlicht in: | European journal of plant pathology 2011-10, Vol.131 (2), p.261-268 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | A 4.1 kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen
Diaporthe perjuncta
. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different
Diaporthe
spp. The transfected species included three
D. ambigua
isolates and an unidentified
Phomopsis
asexual state of a
Diaporthe
sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DRV1 did not reduce growth rate in two of the three
D. ambigua
or the
Phomopsis
sp. significantly. Pathogenicity studies showed that the transfected isolates have reduced aggresiveness. |
---|---|
ISSN: | 0929-1873 1573-8469 |
DOI: | 10.1007/s10658-011-9804-4 |