Four weeks one-leg training and high fat diet does not alter PPARα protein or mRNA expression in human skeletal muscle

Fatty acid metabolism is influenced by training and diet with exercise training mediating this through activation of nuclear hormone receptor peroxisome proliferator-activated receptor alpha (PPAR alpha ) in skeletal muscle. This study investigated the effect of training and high fat or normal diet on PPAR alpha expression in human skeletal muscle. Thirteen men trained one leg (T) four weeks (31.5h in total), while the other leg (UT) served as control. During the 4weeks six subjects consumed high fat (FAT) diet and seven subjects maintained a normal (CHO) diet. Biopsies were obtained from vastus lateralis muscle in both legs before and after training. After the biopsy, one-leg extension exercise was performed in random order with both legs 30min at 95% of workload max. A training effect was evident as citrate synthase activity increased (P