Cloning genes from a library using a clustering strategy and PCR

A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discrim...

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Veröffentlicht in:	Molecular biotechnology 2004, Vol.26 (1), p.35-38
Hauptverfasser:	DIAZ PRADO, S, TARRIO, N, CERDAN, M. E, GONZALEZ SISO, M. I
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cloning Cloning, Molecular Cluster analysis Escherichia coli - metabolism Ethidium - pharmacology Fundamental and applied biological sciences. Psychology Gene Library Genes Genetic Techniques Intercalating Agents - pharmacology Kluyveromyces - metabolism Nucleic Acid Hybridization Plasmids - metabolism Polymerase Chain Reaction Sepharose - chemistry
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Beschreibung
Zusammenfassung:	A new polymerase chain reaction (PCR)-based method is described for the isolation of clones of interest from a library when only part of a sequence is available. In actuality, this occurs with many genomes that have been partially sequenced using a random strategy. The method presented here, discriminating clusters by PCR (DCbyPCR), is a nonradioactive and improved alternative to colony hybridization.
ISSN:	1073-6085 1559-0305 1073-6085
DOI:	10.1385/MB:26:1:35