solid-phase synthesis of 2-5-linked oligoriboadenylates containing 8-bromoadenine
To increase the accessibility of 8-bromo-2′,5′-oligoadenylates, we developed a synthesis of 2′-5′-linked oligoriboadenylates containing varying numbers of 8-bromoadenosine residues based on the use of a CPG-LCA solid support and the phosphoramidite approach. Although N⁶benzoyl protection was satisfa...
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Veröffentlicht in: | Applied biochemistry and biotechnology 1997-07, Vol.67 (1-2), p.33-44 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | To increase the accessibility of 8-bromo-2′,5′-oligoadenylates, we developed a synthesis of 2′-5′-linked oligoriboadenylates containing varying numbers of 8-bromoadenosine residues based on the use of a CPG-LCA solid support and the phosphoramidite approach. Although N⁶benzoyl protection was satisfactory for incorporation of nonmodified adenine residues into 2′,5′-oligonucleotides, the effective incorporation of 8-bromoadenine into such 2′,5′-linked oligomers required use of a non acyl protecting group. Amidine protection of the purine exocyclic amino function proved compatible with all aspects of the phophoramidite approach and with the hydroxyl protection groups employed. |
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ISSN: | 0273-2289 1559-0291 |
DOI: | 10.1007/BF02787839 |