CspC regulates rpoS transcript levels and complements hfq deletions
The general stress response in Escherichia coli is activated by several stress agents, including entering the stationary growth phase. This response constitutes a complex regulatory network in which a large number of genes are induced and others are repressed. The stress response is regulated by the...
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Veröffentlicht in: | Research in microbiology 2010-10, Vol.161 (8), p.694-700 |
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Sprache: | eng |
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Zusammenfassung: | The general stress response in
Escherichia coli is activated by several stress agents, including entering the stationary growth phase. This response constitutes a complex regulatory network in which a large number of genes are induced and others are repressed. The stress response is regulated by the alternative sigma factor σ
S encoded by the
rpoS gene. The
rpoS transcripts are substrates of the RNA binding protein, Hfq, which is essential for its translation. The
rpoS mRNA is also a substrate of the cold shock protein C (CspC) which stabilizes the transcripts. Here we demonstrate, using pull-down assays, that CspC interacts with Hfq via mRNA molecules. We also show that CspC acts on the 5′ UTR of the
rpoS transcript, but its activity on
rpoS is independent of Hfq. Moreover, we show that CspC suppresses the phenotypes of an
hfq deletion. These results elucidate a new aspect in the post-transcriptional regulation of the stress response and will further our understanding of this complex network. |
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ISSN: | 0923-2508 1769-7123 |
DOI: | 10.1016/j.resmic.2010.06.009 |