Improved activity in acidic media of immobilized lysozyme

Polyethylene glycols (PEGs) of various chain length were used to crosslink lysozyme onto an insoluble support such as oxirane. A very high degree of modification and no inactivation of lysozyme were obtained with PEG 20000, but enzymatic activity increased up to 20 times at pH 3.0, at which point th...

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Veröffentlicht in:	Applied biochemistry and biotechnology 1999-03, Vol.76 (3), p.171-181
Hauptverfasser:	ZACCHIGNA, M, DI LUCA, G, LASSIANI, L, VARNAVAS, A, PITOTTI, A, BOCCU, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and medical sciences Biotechnology Enzymatic activity Enzymes Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Leuconostoc oenus Methods. Procedures. Technologies Proteins Studies
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Polyethylene glycols (PEGs) of various chain length were used to crosslink lysozyme onto an insoluble support such as oxirane. A very high degree of modification and no inactivation of lysozyme were obtained with PEG 20000, but enzymatic activity increased up to 20 times at pH 3.0, at which point the activity of the native enzyme was lower when using Leuconostok oenus as a macromolecular substrate.
ISSN:	0273-2289 1559-0291 0273-2289
DOI:	10.1385/ABAB:76:3:171