A lipocalin sequence signature modulates cell survival

Lipocalins are β-barrel proteins, which share three conserved motifs in their amino acid sequence. In this study, we identified by a peptide mapping approach, a seven-amino acid sequence related to one of these motifs (motif 2) that modulates cell survival. A synthetic peptide based on an insect lip...

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Veröffentlicht in:	FEBS letters 2010-07, Vol.584 (13), p.2896-2900
Hauptverfasser:	Chudzinski-Tavassi, Ana Marisa, Carrijo-Carvalho, Linda C., Waismam, Kaline, Farsky, Sandra H.P., Ramos, Oscar H.P., Reis, Cleyson V.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-(4,5-dimethylthiazol-2-methyl)-2,5-diphenyltetrazolium bromide Amino Acid Motifs Animals Apoptosis Apoptosis - drug effects Cell survival Cell Survival - drug effects Cells, Cultured Endothelial Cells - cytology Endothelial Cells - drug effects FBS fetal bovine serum human umbilical vein endothelial cells Humans HUVECs l-NAME Lipocalin Lipocalins - chemistry Lonomia obliqua prothrombin activator protease Lopap MTT nitric oxide Nitric Oxide Synthase - metabolism Nω-nitro-l-arginine methyl ester Peptides - chemical synthesis Peptides - chemistry Peptides - pharmacology Rats relative surface accessibility Roswell Park Memorial Institute 1640 medium RPMI RSA Sequence motif
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Zusammenfassung:	Lipocalins are β-barrel proteins, which share three conserved motifs in their amino acid sequence. In this study, we identified by a peptide mapping approach, a seven-amino acid sequence related to one of these motifs (motif 2) that modulates cell survival. A synthetic peptide based on an insect lipocalin displayed cytoprotective activity in serum-deprived endothelial cells and leucocytes. This activity was dependent on nitric oxide synthase. This sequence was found within several lipocalins, including apolipoprotein D, retinol binding protein, lipocalin-type prostaglandin D synthase, and many unknown proteins, suggesting that it is a sequence signature and a lipocalin conserved property.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2010.05.008