Targeted cyst wall puncture and aspiration during EUS-FNA increases the diagnostic yield of premalignant and malignant pancreatic cysts

Background Characterization of pancreatic cysts by using EUS-FNA includes chemical and cytologic analysis. Objective To evaluate whether material obtained from FNA of the cyst wall increases diagnostic yield. Design Prospective series. Setting Tertiary referral center. Patients Consecutive patients with pancreatic cysts referred for EUS-FNA between March 2010 and March 2011. Intervention FNA was performed with aspiration of cyst fluid for carcinoembryonic antigen (CEA) and cytology, followed by cyst wall puncture (CWP). CWP is defined as puncturing the far wall of the cyst and moving the needle back and forth through the wall to sample the wall epithelium. Main Outcome Measurements The diagnostic yield for mucinous cystic pancreatic neoplasms by CEA and cytology obtained from cyst fluid compared with cytology obtained from CWP. CEA ≥192 ng/mL was considered mucinous. Results A total of 69 pancreatic cysts from 66 patients were included. Adequate amounts of fluid were aspirated for CEA, amylase, and cytology in 60 cysts (81%). Cellular material adequate for cytologic assessment from CWP was obtained in 56 cysts (81%). Ten (30%) of 33 cysts with CEA