Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass
The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme was purified to homogeneity by anion-exchange and hy...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2011-12, Vol.38 (12), p.1961-1967 |
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| container_end_page | 1967 |
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| container_issue | 12 |
| container_start_page | 1961 |
| container_title | Journal of industrial microbiology & biotechnology |
| container_volume | 38 |
| creator | Wong, Dominic W. S Chan, Victor J Batt, Sarah B Sarath, Gautam Liao, Hans |
| description | The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography. The specific activity was determined to be 8,200 U/μg (pH 6.5, 20°C, 3.5 mM 4-nitrophenyl ferulate). The protein had a correct N-terminal sequence of ASTQGISEDLY, indicating that the signal peptide was properly processed. The FAE exhibited an optimum pH of 6–7 and operated optimally at 50°C using ground switchgrass as the substrate. The yeast clone was demonstrated to catalyze the release of ferulic acid continuously from switchgrass in YNB medium at 30°C. This work represents the first report on engineering yeast for the breakdown of ferulic acid crosslink to facilitate consolidated bioprocessing. |
| doi_str_mv | 10.1007/s10295-011-0985-9 |
| format | Article |
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S ; Chan, Victor J ; Batt, Sarah B ; Sarath, Gautam ; Liao, Hans</creator><creatorcontrib>Wong, Dominic W. S ; Chan, Victor J ; Batt, Sarah B ; Sarath, Gautam ; Liao, Hans</creatorcontrib><description>The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography. The specific activity was determined to be 8,200 U/μg (pH 6.5, 20°C, 3.5 mM 4-nitrophenyl ferulate). The protein had a correct N-terminal sequence of ASTQGISEDLY, indicating that the signal peptide was properly processed. The FAE exhibited an optimum pH of 6–7 and operated optimally at 50°C using ground switchgrass as the substrate. The yeast clone was demonstrated to catalyze the release of ferulic acid continuously from switchgrass in YNB medium at 30°C. This work represents the first report on engineering yeast for the breakdown of ferulic acid crosslink to facilitate consolidated bioprocessing.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-011-0985-9</identifier><identifier>PMID: 21626208</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>anion exchange ; Aspergillus niger ; Aspergillus niger - enzymology ; Aspergillus niger - genetics ; Biochemistry ; Bioinformatics ; Biological and medical sciences ; Biomass ; Biomedical and Life Sciences ; bioprocessing ; Biotechnology ; Carboxylic Ester Hydrolases - metabolism ; Catalysis ; chromatography ; Cloning ; Coumaric Acids - isolation & purification ; Engineering ; Enzyme kinetics ; Enzymes ; ferulic acid ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic Engineering ; Glycerol ; Grasses ; hydrophobic bonding ; Inorganic Chemistry ; Kinases ; Life Sciences ; Lignin ; Microbiology ; Nitro Compounds ; Original Paper ; Panicum - chemistry ; Panicum virgatum ; Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; secretion ; signal peptide ; Studies ; Yeast ; Yeasts</subject><ispartof>Journal of industrial microbiology & biotechnology, 2011-12, Vol.38 (12), p.1961-1967</ispartof><rights>Springer-Verlag (outside the USA) 2011</rights><rights>2015 INIST-CNRS</rights><rights>Society for Industrial Microbiology and Biotechnology 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-c978f70b505c73256aac9a38563cb25510efb695fbc101dda8fee6a205487503</citedby><cites>FETCH-LOGICAL-c499t-c978f70b505c73256aac9a38563cb25510efb695fbc101dda8fee6a205487503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-011-0985-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-011-0985-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25262536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21626208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wong, Dominic W. S</creatorcontrib><creatorcontrib>Chan, Victor J</creatorcontrib><creatorcontrib>Batt, Sarah B</creatorcontrib><creatorcontrib>Sarath, Gautam</creatorcontrib><creatorcontrib>Liao, Hans</creatorcontrib><title>Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography. The specific activity was determined to be 8,200 U/μg (pH 6.5, 20°C, 3.5 mM 4-nitrophenyl ferulate). The protein had a correct N-terminal sequence of ASTQGISEDLY, indicating that the signal peptide was properly processed. The FAE exhibited an optimum pH of 6–7 and operated optimally at 50°C using ground switchgrass as the substrate. The yeast clone was demonstrated to catalyze the release of ferulic acid continuously from switchgrass in YNB medium at 30°C. This work represents the first report on engineering yeast for the breakdown of ferulic acid crosslink to facilitate consolidated bioprocessing.</description><subject>anion exchange</subject><subject>Aspergillus niger</subject><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - genetics</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>bioprocessing</subject><subject>Biotechnology</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Catalysis</subject><subject>chromatography</subject><subject>Cloning</subject><subject>Coumaric Acids - isolation & purification</subject><subject>Engineering</subject><subject>Enzyme kinetics</subject><subject>Enzymes</subject><subject>ferulic acid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetic Engineering</subject><subject>Glycerol</subject><subject>Grasses</subject><subject>hydrophobic bonding</subject><subject>Inorganic Chemistry</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Lignin</subject><subject>Microbiology</subject><subject>Nitro Compounds</subject><subject>Original Paper</subject><subject>Panicum - chemistry</subject><subject>Panicum virgatum</subject><subject>Proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>secretion</subject><subject>signal peptide</subject><subject>Studies</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkktv1DAUhSMEoqXwA9iAhYRYBa7tXDteoqo8pEosWtaR41zPuMrEgz0Bzb_HIQOVWMDKD33n3GMdV9VzDm85gH6XOQiDNXBeg2mxNg-qc95oVSNKfFj2UukaG4ln1ZOc7wAAtRaPqzPBlVAC2vMqXU2bMBGlMG3YjXVua1PcHR1l5ijR95CDJXaIbJ_iMDtintI8xuPIKB8o2VxuYmKHLbFEIy3n6FcoOGZdGJgvhiz_CAe33RRBflo98nbM9Oy0XlS3H65uLz_V118-fr58f127xphD7YxuvYYeAZ2WApW1zljZopKuF4gcyPfKoO8dBz4MtvVEygrAptUI8qJ6s9qW5N_mkrbbhexoHO1Ecc6dEcqAlLr5PwnYag6_PF_9Rd7FOU3lFQskhGn4AvEVcinmnMh3-xR2Nh07Dt3SW7f21pXeuqW3zhTNi5Px3O9o-KP4XVQBXp8Am50dfbKTC_mew4KhVIUTK5f3S6WU7hP-a_rLVeRt7OwmFeOvNwJ4s_wYCbyVPwErhrmH</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Wong, Dominic W. 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Psychology</topic><topic>genes</topic><topic>Genetic Engineering</topic><topic>Glycerol</topic><topic>Grasses</topic><topic>hydrophobic bonding</topic><topic>Inorganic Chemistry</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Lignin</topic><topic>Microbiology</topic><topic>Nitro Compounds</topic><topic>Original Paper</topic><topic>Panicum - chemistry</topic><topic>Panicum virgatum</topic><topic>Proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>secretion</topic><topic>signal peptide</topic><topic>Studies</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, Dominic W. 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S</au><au>Chan, Victor J</au><au>Batt, Sarah B</au><au>Sarath, Gautam</au><au>Liao, Hans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>38</volume><issue>12</issue><spage>1961</spage><epage>1967</epage><pages>1961-1967</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>The Aspergillus niger feruloyl esterase gene (faeA) was cloned into Saccharomyces cerevisiae via a yeast expression vector, resulting in efficient expression and secretion of the enzyme in the medium with a yield of ~2 mg/l. The recombinant enzyme was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography. The specific activity was determined to be 8,200 U/μg (pH 6.5, 20°C, 3.5 mM 4-nitrophenyl ferulate). The protein had a correct N-terminal sequence of ASTQGISEDLY, indicating that the signal peptide was properly processed. The FAE exhibited an optimum pH of 6–7 and operated optimally at 50°C using ground switchgrass as the substrate. The yeast clone was demonstrated to catalyze the release of ferulic acid continuously from switchgrass in YNB medium at 30°C. This work represents the first report on engineering yeast for the breakdown of ferulic acid crosslink to facilitate consolidated bioprocessing.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21626208</pmid><doi>10.1007/s10295-011-0985-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | anion exchange Aspergillus niger Aspergillus niger - enzymology Aspergillus niger - genetics Biochemistry Bioinformatics Biological and medical sciences Biomass Biomedical and Life Sciences bioprocessing Biotechnology Carboxylic Ester Hydrolases - metabolism Catalysis chromatography Cloning Coumaric Acids - isolation & purification Engineering Enzyme kinetics Enzymes ferulic acid Fundamental and applied biological sciences. Psychology genes Genetic Engineering Glycerol Grasses hydrophobic bonding Inorganic Chemistry Kinases Life Sciences Lignin Microbiology Nitro Compounds Original Paper Panicum - chemistry Panicum virgatum Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics secretion signal peptide Studies Yeast Yeasts |
| title | Engineering Saccharomyces cerevisiae to produce feruloyl esterase for the release of ferulic acid from switchgrass |
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