A fast and easy real-time PCR genotyping method for the HLA-G 14-bp insertion/deletion polymorphism in the 3′ untranslated region

Human leukocyte antigen‐G (HLA‐G) is a non‐classical HLA class Ib molecule shown to exhibit immunomodulatory function in a wide range of immune‐based disorders. A number of functional HLA‐G gene polymorphisms have been identified, including a 14‐bp insertion/deletion polymorphism in exon 8 of the 3′ untranslated region of the HLA‐G gene, which has been associated with HLA‐G mRNA stability. Moreover, studies show that homozygosity for the 14‐bp insertion/deletion polymorphism is associated with lower HLA‐G mRNA and protein levels and unique alternative splicing patterns. Here, we introduce a quick and reliable method to screen for the HLA‐G 14‐bp insertion/deletion polymorphism using an optimized real‐time polymerase chain reaction protocol. The genotyping assay has been validated by comparison with conventional methods. As results can be obtained within a few hours, the assay will have a potential for clinical use.