Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells

We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological...

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Veröffentlicht in:Neurophysiology (New York) 2011-12, Vol.43 (4), p.321-323
Hauptverfasser: Dumanskaya, G. V., Purnyn, H. E., Rykhal’skii, O. V., Veselovskii, N. S.
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container_issue 4
container_start_page 321
container_title Neurophysiology (New York)
container_volume 43
creator Dumanskaya, G. V.
Purnyn, H. E.
Rykhal’skii, O. V.
Veselovskii, N. S.
description We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies.
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subjects Biomedical and Life Sciences
Biomedicine
Cell culture
Ganglion
Neurosciences
Retina
Retinal ganglion cells
Rodents
title Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells
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