Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells
We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological...
Gespeichert in:
Veröffentlicht in: | Neurophysiology (New York) 2011-12, Vol.43 (4), p.321-323 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 323 |
---|---|
container_issue | 4 |
container_start_page | 321 |
container_title | Neurophysiology (New York) |
container_volume | 43 |
creator | Dumanskaya, G. V. Purnyn, H. E. Rykhal’skii, O. V. Veselovskii, N. S. |
description | We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies. |
doi_str_mv | 10.1007/s11062-011-9223-3 |
format | Article |
fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_926881879</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A358631007</galeid><sourcerecordid>A358631007</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-6260191058da1463b3f635da9e81d6cb7c7c9b29944768ccb5cdb1acc350d3813</originalsourceid><addsrcrecordid>eNp1kk9rFTEUxQdR8Fn9AO6CLqSL1PyZySTuymhr4UHLU9chk2TGlHnJM8mAXfnVm-kU5Ikli4Sb3zncy7lV9RajM4xQ-zFhjBiBCGMoCKGQPqs2uGkpFOX3ebVBSCBIRNu-rF6ldIsQYlw0m-rPTXR7Fe9AN095jhaEAXx2KQXtVLYGdHaa0lLMPy3YqQx2NjuvwOyNjaAL3rjsgn9AtsGPcKtSAcZHv_L6BG5iONiYnX2gLpUfpyJZrV9XLwY1Jfvm8T6pflx8-d59hdvry6vufAt1XfMMGWEIC4wabhSuGe3pwGhjlLAcG6b7Vrda9ESIum4Z17pvtOmx0po2yFCO6Un1YfU9xPBrtinLvUu6dKC8DXOSgjDOMW9FId_9Q96GOfrSnBSIsbqpyQK9X6FRTVY6P4QclV4s5TltOKNLKIU6-w9VjrF7p4O3gyv1I8HpkaAw2f7Oo5pTklffdscsXlkdQ0rRDvKwRikxkgsh152QZSfkshOSFg1ZNemwRGPj39meFt0De562_w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>906645429</pqid></control><display><type>article</type><title>Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells</title><source>SpringerLink Journals</source><creator>Dumanskaya, G. V. ; Purnyn, H. E. ; Rykhal’skii, O. V. ; Veselovskii, N. S.</creator><creatorcontrib>Dumanskaya, G. V. ; Purnyn, H. E. ; Rykhal’skii, O. V. ; Veselovskii, N. S.</creatorcontrib><description>We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies.</description><identifier>ISSN: 0090-2977</identifier><identifier>EISSN: 1573-9007</identifier><identifier>DOI: 10.1007/s11062-011-9223-3</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell culture ; Ganglion ; Neurosciences ; Retina ; Retinal ganglion cells ; Rodents</subject><ispartof>Neurophysiology (New York), 2011-12, Vol.43 (4), p.321-323</ispartof><rights>Springer Science+Business Media, Inc. 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-6260191058da1463b3f635da9e81d6cb7c7c9b29944768ccb5cdb1acc350d3813</citedby><cites>FETCH-LOGICAL-c448t-6260191058da1463b3f635da9e81d6cb7c7c9b29944768ccb5cdb1acc350d3813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11062-011-9223-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11062-011-9223-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Dumanskaya, G. V.</creatorcontrib><creatorcontrib>Purnyn, H. E.</creatorcontrib><creatorcontrib>Rykhal’skii, O. V.</creatorcontrib><creatorcontrib>Veselovskii, N. S.</creatorcontrib><title>Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells</title><title>Neurophysiology (New York)</title><addtitle>Neurophysiology</addtitle><description>We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell culture</subject><subject>Ganglion</subject><subject>Neurosciences</subject><subject>Retina</subject><subject>Retinal ganglion cells</subject><subject>Rodents</subject><issn>0090-2977</issn><issn>1573-9007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kk9rFTEUxQdR8Fn9AO6CLqSL1PyZySTuymhr4UHLU9chk2TGlHnJM8mAXfnVm-kU5Ikli4Sb3zncy7lV9RajM4xQ-zFhjBiBCGMoCKGQPqs2uGkpFOX3ebVBSCBIRNu-rF6ldIsQYlw0m-rPTXR7Fe9AN095jhaEAXx2KQXtVLYGdHaa0lLMPy3YqQx2NjuvwOyNjaAL3rjsgn9AtsGPcKtSAcZHv_L6BG5iONiYnX2gLpUfpyJZrV9XLwY1Jfvm8T6pflx8-d59hdvry6vufAt1XfMMGWEIC4wabhSuGe3pwGhjlLAcG6b7Vrda9ESIum4Z17pvtOmx0po2yFCO6Un1YfU9xPBrtinLvUu6dKC8DXOSgjDOMW9FId_9Q96GOfrSnBSIsbqpyQK9X6FRTVY6P4QclV4s5TltOKNLKIU6-w9VjrF7p4O3gyv1I8HpkaAw2f7Oo5pTklffdscsXlkdQ0rRDvKwRikxkgsh152QZSfkshOSFg1ZNemwRGPj39meFt0De562_w</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Dumanskaya, G. V.</creator><creator>Purnyn, H. E.</creator><creator>Rykhal’skii, O. V.</creator><creator>Veselovskii, N. S.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope></search><sort><creationdate>20111201</creationdate><title>Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells</title><author>Dumanskaya, G. V. ; Purnyn, H. E. ; Rykhal’skii, O. V. ; Veselovskii, N. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-6260191058da1463b3f635da9e81d6cb7c7c9b29944768ccb5cdb1acc350d3813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell culture</topic><topic>Ganglion</topic><topic>Neurosciences</topic><topic>Retina</topic><topic>Retinal ganglion cells</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dumanskaya, G. V.</creatorcontrib><creatorcontrib>Purnyn, H. E.</creatorcontrib><creatorcontrib>Rykhal’skii, O. V.</creatorcontrib><creatorcontrib>Veselovskii, N. S.</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><jtitle>Neurophysiology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dumanskaya, G. V.</au><au>Purnyn, H. E.</au><au>Rykhal’skii, O. V.</au><au>Veselovskii, N. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells</atitle><jtitle>Neurophysiology (New York)</jtitle><stitle>Neurophysiology</stitle><date>2011-12-01</date><risdate>2011</risdate><volume>43</volume><issue>4</issue><spage>321</spage><epage>323</epage><pages>321-323</pages><issn>0090-2977</issn><eissn>1573-9007</eissn><abstract>We developed a technique for long-lasting culturing of dissociated cells of the rat retina. Electrophysiological characteristics of cultured retinal ganglion cells were examined using a patch-clamp technique in the whole-cell configuration (current-clamp mode). Morphological and electrophysiological characteristics of cultured retinal ganglion cells corresponded to those obtained under conditions of an acute experiment on the cells of similar-age animals; this is indicative of the possibility of using cultured retinal cells in electrophysiological and pharmacological studies.</abstract><cop>Boston</cop><pub>Springer US</pub><doi>10.1007/s11062-011-9223-3</doi><tpages>3</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0090-2977 |
ispartof | Neurophysiology (New York), 2011-12, Vol.43 (4), p.321-323 |
issn | 0090-2977 1573-9007 |
language | eng |
recordid | cdi_proquest_miscellaneous_926881879 |
source | SpringerLink Journals |
subjects | Biomedical and Life Sciences Biomedicine Cell culture Ganglion Neurosciences Retina Retinal ganglion cells Rodents |
title | Primary Culture of Dissociated Cells of the Rat Retina under Conditions of Long-Lasting Culturing: Properties of Ganglion Cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T13%3A45%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Primary%20Culture%20of%20Dissociated%20Cells%20of%20the%20Rat%20Retina%20under%20Conditions%20of%20Long-Lasting%20Culturing:%20Properties%20of%20Ganglion%20Cells&rft.jtitle=Neurophysiology%20(New%20York)&rft.au=Dumanskaya,%20G.%20V.&rft.date=2011-12-01&rft.volume=43&rft.issue=4&rft.spage=321&rft.epage=323&rft.pages=321-323&rft.issn=0090-2977&rft.eissn=1573-9007&rft_id=info:doi/10.1007/s11062-011-9223-3&rft_dat=%3Cgale_proqu%3EA358631007%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=906645429&rft_id=info:pmid/&rft_galeid=A358631007&rfr_iscdi=true |