Antigen-specific responses assessment for the evaluation of Bordetella pertussis T cell immunity in humans

Highlights ► The assessment of T cell responses is technically complex and usually performed on fresh PBMC. ► To identify simplified methods to assess pertussis specific T cell responses. ► High concordance in proliferation results was obtained when CFSE and blast read-outs were compared. ► Pertussi...

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Veröffentlicht in:	Vaccine 2012-02, Vol.30 (9), p.1667-1674
Hauptverfasser:	Carollo, Maria, Palazzo, Raffaella, Bianco, Manuela, Smits, Kaatje, Mascart, Françoise, Ausiello, Clara Maria
Format:	Artikel
Sprache:	eng
Schlagworte:	3H-thymidine incorporation 5,6-Carboxylfluorescein diacetate succinimidyl ester (CFSE) analysis Adult Allergy and Immunology Antigens, Bacterial - immunology Blasts analysis Bordetella pertussis Bordetella pertussis - immunology Cell Culture Techniques Cell Proliferation Cells, Cultured Cryopreservation Flow Cytometry fluorescence Humans immunity Interferon-gamma - immunology Leukocytes, Mononuclear - immunology Lymphocyte Activation Pertussis Pertussis Toxin - immunology radiolabeling T cell responses T-lymphocytes T-Lymphocytes - immunology t-test Th1 cytokines (IFNγ, TNFα) Thymidine - metabolism tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - immunology vaccination vaccines
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Beschreibung
Zusammenfassung:	Highlights ► The assessment of T cell responses is technically complex and usually performed on fresh PBMC. ► To identify simplified methods to assess pertussis specific T cell responses. ► High concordance in proliferation results was obtained when CFSE and blast read-outs were compared. ► Pertussis-specific assays performed on fresh PBMC were compared to frozen PBMC from the same donor. ► Results from fresh and frozen PBMC did not show significant difference. ► Adopting blast analysis and frozen PBMC, the possibility to test T cell responses is simplified.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2011.12.104