CD205 antigen targeting combined with dendritic cell recruitment factors and antigen-linked CD40L activation primes and expands significant antigen-specific antibody and CD4+ T cell responses following DNA vaccination of outbred animals

Highlights ► We tested immunogenicity of a CD205-targeted antigen fused to CD40L. ► We tested effectiveness of a single low dose. ► Immunization of MHC class II-matched calves induced significant immune responses. ► Primed immune responses were significantly expanded three weeks post-immunization. ►...

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Veröffentlicht in:	Vaccine 2012-02, Vol.30 (9), p.1624-1635
Hauptverfasser:	Njongmeta, Leo M, Bray, Jocelyn, Davies, Christopher J, Davis, William C, Howard, Chris J, Hope, Jayne C, Palmer, Guy H, Brown, Wendy C, Mwangi, Waithaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Anaplasma marginale - immunology animal models Animals Antibody Formation Antigen-targeting antigens Antigens, CD - immunology B-Lymphocytes - immunology Bacterial Outer Membrane Proteins - immunology calves Cattle CD4 T cells CD4-positive T-lymphocytes CD4-Positive T-Lymphocytes - immunology CD40 Ligand - immunology CD40L Cell growth Cell Proliferation Dendritic cell Dendritic Cells - immunology Deoxyribonucleic acid DNA DNA vaccine Dose-Response Relationship, Immunologic granulocyte-macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - immunology HEK293 Cells hosts Humans immune response Immune system Immunization Immunogenicity immunoglobulin G Immunoglobulin G - blood Interferon-gamma - immunology Intradermal Lectins, C-Type - immunology Linked activation Lymphocyte Activation Lymphocytes Male Medical research Membrane Proteins - immunology Minor Histocompatibility Antigens Outbred species Receptors, Cell Surface - immunology recruitment Studies T cell receptors vaccination Vaccines Vaccines, DNA - immunology
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Beschreibung
Zusammenfassung:	Highlights ► We tested immunogenicity of a CD205-targeted antigen fused to CD40L. ► We tested effectiveness of a single low dose. ► Immunization of MHC class II-matched calves induced significant immune responses. ► Primed immune responses were significantly expanded three weeks post-immunization. ► Mean immune responses tripled within one week post-boost.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2011.12.110