Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

We present a disposable microarray hybridization chamber with an integrated micropump to speed up diffusion based reaction kinetics by generating convective flow. The time-to-result for the hybridization reaction was reduced from 60 min (standard protocol) down to 15 min for a commercially available...

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Veröffentlicht in:	Lab on a chip 2012-04, Vol.12 (7), p.1384-1388
Hauptverfasser:	Rupp, Jochen, Schmidt, Manuela, Münch, Susanne, Cavalar, Markus, Steller, Ulf, Steigert, Jürgen, Stumber, Michael, Dorrer, Christian, Rothacher, Peter, Zengerle, Roland, Daub, Martina
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA - metabolism Fluorescent Dyes - chemistry Kinetics Membranes, Artificial Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Polycarboxylate Cement - chemistry
Online-Zugang:	Volltext
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