The Antioxidative Activity of Riboflavin in the Presence of Antipyrin. Spectroscopic Studies

The effect of Antipyrin upon the antioxidant activity of the riboflavin has been evidenced using chemiluminescent system luminol–hydrogen peroxide, in Tris–HCl buffer, pH 8.5. It was found that riboflavin antioxidant activity depends on the reaction time and the Antipyrin concentration. Using ESR spectroscopy the hydroxyl radical generation, in the mentioned chemiluminescent system, was evidenced. The interaction between reactants was also investigated by UV-VIS and fluorescence spectroscopy. The effect of Antipyrin concentration upon the riboflavin fluorescence has also been investigated. The fluorescence quenching by Antipyrin is not significant and subsequently the riboflavin fluorescence quenching doesn’t indicate an electron transfer process through diffusion-controlled mechanism. The results are discussed with relevance to the redox processes of riboflavin.