Caged glucosamine-6-phosphate for the light-control of riboswitch activity

We have synthesized a light-activatable (“caged”) derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3′-untranslated region of eukaryotic mRNA molecules and caged Glc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Photochemical & photobiological sciences 2012-03, Vol.11 (3), p.489-492
Hauptverfasser:	Wulffen, Bernhard, Buff, Maximilian Clemens Rudolf, Pofahl, Monika, Mayer, Günter, Heckel, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biomaterials Chemistry Communication Glucosamine - analogs & derivatives Glucosamine - chemical synthesis Glucosamine - chemistry Glucosamine - metabolism Glucose-6-Phosphate - analogs & derivatives Glucose-6-Phosphate - chemical synthesis Glucose-6-Phosphate - chemistry Glucose-6-Phosphate - metabolism Light Physical Chemistry Plant Sciences Riboswitch RNA, Catalytic - chemistry RNA, Catalytic - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We have synthesized a light-activatable (“caged”) derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3′-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro .
ISSN:	1474-905X 1474-9092
DOI:	10.1039/c1pp05242j