Enzyme-free signal amplification in the DNAzyme sensor via target-catalyzed hairpin assembly

A simple, highly sensitive and enzyme-free DNAzyme sensor based on target-catalyzed hairpin assembly is developed, which permits detection of 0.1 pM target DNA. Furthermore, this DNAzyme sensor is capable of detecting target DNA in real samples because of its high selectivity.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2012-03, Vol.48 (25), p.3112-3114
Hauptverfasser:	Zheng, Ai-Xian, Li, Juan, Wang, Jin-Ru, Song, Xiao-Rong, Chen, Guo-Nan, Yang, Huang-Hao
Format:	Artikel
Sprache:	eng
Schlagworte:	Biocatalysis Biosensing Techniques - methods DNA - analysis DNA - chemistry DNA, Catalytic Nucleic Acid Conformation Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	A simple, highly sensitive and enzyme-free DNAzyme sensor based on target-catalyzed hairpin assembly is developed, which permits detection of 0.1 pM target DNA. Furthermore, this DNAzyme sensor is capable of detecting target DNA in real samples because of its high selectivity.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c2cc30305a