Development of quantitative duplex real-time PCR method for screening analysis of genetically modified maize

A duplex real-time PCR method was developed for quantitative screening analysis of GM maize. The duplex real-time PCR simultaneously detected two GM-specific segments, namely the cauliflower mosaic virus (CaMV) 35S promoter (P35S) segment and an event-specific segment for GA21 maize which does not c...

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Veröffentlicht in:	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2009/06/25, Vol.50(3), pp.117-125
Hauptverfasser:	Oguchi, T.(National Food Research Inst., Tsukuba, Ibaraki (Japan)), Onishi, M, Minegishi, Y, Kurosawa, Y, Kasahara, M, Akiyama, H, Teshima, R, Futo, S, Furui, S, Hino, A, Kitta, K
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE ANALYTICAL METHODS Cauliflower mosaic virus CONCEPTION DESIGN DISENO DNA duplex real-time PCR EXTRACCION EXTRACTION Food Analysis - methods Food, Genetically Modified genetically modified (GM) GENETICALLY MODIFIED ORGANISMS GRAINE MAIS MAIZ MAIZE maize (Zea mays) ORGANISME GENETIQUEMENT MODIFIE ORGANISMOS MODIFICADOS GENETICAMENTE PCR PLASMIDE PLASMIDIOS PLASMIDS Polymerase Chain Reaction - methods QUANTITATIVE ANALYSIS SEEDS SEMILLA TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Zea mays Zea mays - genetics
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