Interlaboratory validation of an event-specific real time polymerase chain reaction detection method for genetically modified DAS59132 maize

A real-time polymerase chain reaction (PCR) method specific for genetically modified (GM) maize event DAS59132 (E32) was adapted for qualitative detection of low level presence of E32. The method was validated by a collaborative trial with eight participating Japanese laboratories. Sensitivity was a...

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Veröffentlicht in:	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2010/04/25, Vol.51(2), pp.65-70
Hauptverfasser:	Akiyama, H., National Inst. of Health Sciences, Tokyo (Japan), Sakata, K, Spiegelhalter, F, Furui, S, Nakashima, A, Kitta, K, Teshima, R
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ADN RECOMBINADO ADN RECOMBINE ANALISIS CUALITATIVO ANALYSE QUALITATIVE ANALYTICAL METHODS DAS59132 detection method DNA DNA, Plant Food Analysis - methods Food Contamination - analysis Food, Genetically Modified genetically modified maize GENETICALLY MODIFIED ORGANISMS http://www.fao.org/aos/agrovoc#c_12332 http://www.fao.org/aos/agrovoc#c_1513 http://www.fao.org/aos/agrovoc#c_2347 http://www.fao.org/aos/agrovoc#c_27594 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_34285 http://www.fao.org/aos/agrovoc#c_35284 http://www.fao.org/aos/agrovoc#c_6475 MAIS MAIZ MAIZE ORGANISME GENETIQUEMENT MODIFIE ORGANISMOS MODIFICADOS GENETICAMENTE PCR Polymerase Chain Reaction - methods QUALITATIVE ANALYSIS real time PCR RECOMBINACION RECOMBINAISON RECOMBINANT DNA RECOMBINATION Reproducibility of Results Sensitivity and Specificity Solutions TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Zea mays Zea mays - genetics
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Zusammenfassung:	A real-time polymerase chain reaction (PCR) method specific for genetically modified (GM) maize event DAS59132 (E32) was adapted for qualitative detection of low level presence of E32. The method was validated by a collaborative trial with eight participating Japanese laboratories. Sensitivity was assessed with three different samples of corn flour fortified to 0%, 0.05% and 0.1% (w/w) E32 respectively. In addition, a 0.01% E32 DNA solution was used. The detection limit with DNA solution was estimated to be approximately 0.01%. In conclusion, the results of the study confirmed this real-time PCR method as a reliable tool for qualitative detection of E32 maize.
ISSN:	0015-6426 1882-1006
DOI:	10.3358/shokueishi.51.65