PP1-Mediated Moesin Dephosphorylation Couples Polar Relaxation to Mitotic Exit
Animal cells undergo dramatic actin-dependent changes in shape as they progress through mitosis; they round up upon mitotic entry and elongate during chromosome segregation before dividing into two [1–3]. Moesin, the sole Drosophila ERM-family protein [4], plays a critical role in this process, thro...
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Veröffentlicht in: | Current biology 2012-02, Vol.22 (3), p.231-236 |
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Sprache: | eng |
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Zusammenfassung: | Animal cells undergo dramatic actin-dependent changes in shape as they progress through mitosis; they round up upon mitotic entry and elongate during chromosome segregation before dividing into two [1–3]. Moesin, the sole Drosophila ERM-family protein [4], plays a critical role in this process, through the construction of a stiff, rounded metaphase cortex [5–7]. At mitotic exit, this rigid cortex must be dismantled to allow for anaphase elongation and cytokinesis through the loss of the active pool of phospho-Thr559moesin from cell poles. Here, in an RNA interference (RNAi) screen for phosphatases involved in the temporal and spatial control of moesin, we identify PP1-87B RNAi as having elevated p-moesin levels and reduced cortical compliance. In mitosis, RNAi-induced depletion of PP1-87B or depletion of a conserved noncatalytic PP1 phosphatase subunit Sds22 leads to defects in p-moesin clearance from cell poles at anaphase, a delay in anaphase elongation, together with defects in bipolar anaphase relaxation and cytokinesis. Importantly, similar cortical defects are seen at anaphase following the expression of a constitutively active, phosphomimetic version of moesin. These data reveal a new role for the PP1-87B/Sds22 phosphatase, an important regulator of the metaphase-anaphase transition, in coupling moesin-dependent cell shape changes to mitotic exit.
► PP1-87B inhibits moesin phosphorylation, cell rounding, and cortical rigidity ► PP1-87B, Sds22, and moesin inactivation are required for anaphase polar relaxation ► PP1 phosphatase counters Slik to couple anaphase cell elongation to mitotic exit ► Changes in T559-moesin phosphorylation status drive mitotic cell shape dynamics |
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ISSN: | 0960-9822 1879-0445 |
DOI: | 10.1016/j.cub.2011.12.016 |