Identification of differentially expressed genes in ovaries of chicken attaining sexual maturity at different ages

In poultry as well as in other birds, sexual maturity is one of the important factors influencing female reproduction and egg production. In this study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) differential display approach was used to identify genes related to sexual maturity. Using...

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Veröffentlicht in:	Molecular biology reports 2012-03, Vol.39 (3), p.3037-3045
Hauptverfasser:	Kang, Li, Zhang, Yujie, Zhang, Ningbo, Zang, Li, Wang, Meng, Cui, Xinxing, Jiang, Yunliang
Format:	Artikel
Sprache:	eng
Schlagworte:	Age Age Factors Amplified Fragment Length Polymorphism Analysis Analysis of Variance Animal Anatomy Animal Biochemistry Animal reproduction Animals Base Sequence Biomedical and Life Sciences Chickens - genetics Chickens - metabolism China Cloning, Molecular Computational Biology Differential display DNA Primers - genetics DNA, Complementary - genetics Egg production Female Gene expression Gene Expression Profiling Gene Expression Regulation - genetics Gene polymorphism Genes - genetics Histology Life Sciences Molecular Sequence Data Morphology Ovaries Ovary - metabolism Polymerase chain reaction Poultry Primers Real-Time Polymerase Chain Reaction Reproduction Sequence Analysis, DNA Sexual Maturation - genetics Sexual maturity
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Zusammenfassung:	In poultry as well as in other birds, sexual maturity is one of the important factors influencing female reproduction and egg production. In this study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) differential display approach was used to identify genes related to sexual maturity. Using 54 Eco R I/ Mse I selective primer combinations, totally 403 differentially expressed transcript-derived fragments (TDFs) were isolated, 27 of which belong to 25 unigenes. By real-time quantitative PCR (qPCR), the expression pattern of 13 genes was confirmed; among them, four genes including ZNF183 ( P
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-011-1066-x